2. Apoptosis
  3. Apoptosis
  4. BCH

BCH  (Synonyms: 2-Amino-2-norbornanecarboxylic acid; LAT1-IN-1)

Cat. No.: HY-108540 Purity: ≥98.0%
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BCH (2-Amino-2-norbornanecarboxylic acid) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis.

For research use only. We do not sell to patients.

BCH Chemical Structure

BCH Chemical Structure

CAS No. : 20448-79-7

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Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 73 In-stock
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10 mM * 1 mL in Water USD 73 In-stock
Solid
50 mg USD 66 In-stock
100 mg USD 110 In-stock
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Based on 4 publication(s) in Google Scholar

BCH Related Antibodies

Top Publications Citing Use of Products

    BCH purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    LAT1-IN1 (30 nM) inhibits the expression levels of phosphorylated mTOR and P70S6K in T cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BCH (2-Amino-2-norbornanecarboxylic acid) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis[1][2][3].

    IC50 & Target

    LAT1[1]
    In Vitro

    BCH (1-100 mM; 3 days; KYSE30 and KYSE150 esophageal cancer cells) treatment suppresses cell proliferation in a dose-dependent manner[1].
    BCH (30 mM; 24 and 48 hours; KYSE30 and KYSE150 cells) treatment significantly increases cell population in the G0/G1 phase in both KYSE30 and KYSE150 cells, indicating that BCH induces cell cycle arrest at G1 phase[1].
    BCH (30 mM; 0-24 hours; KYSE30 and KYSE150 cells) treatment decreases phosphorylation of 4E-BP1 and p70S6K at 30 minutes and the decrease is continued for 24 hours. The amount of mTOR, 4E-BP1, and p70S6K proteins is slightly decreased[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BCH Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: KYSE30 and KYSE150 esophageal cancer cells
    Concentration: 1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM
    Incubation Time: 3 days
    Result: Cell proliferation was suppressed in a dose-dependent manner.

    Cell Cycle Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 24 and 48 hours
    Result: Cell cycle arrest.

    Western Blot Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours
    Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.
    In Vivo

    BCH (200 mg/kg; intravenous injection; daily; for 14 days; male BALB/c nude mice) treatment significantly delays tumor growth and decreases glucose metabolism, indicating that LAT1 inhibition potentially suppresses esophageal cancer growth in vivo[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BALB/c nude mice (5-week-old) with KYSE150 cells[1]
    Dosage: 200 mg/kg
    Administration: Intravenous injection; daily; for 14 days
    Result: Significantly delayed tumor growth and decreased glucose metabolism.
    Clinical Trial
    Molecular Weight

    155.19

    Formula

    C8H13NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1(N)C(C2)CCC2C1)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 20 mg/mL (128.87 mM; ultrasonic and warming and heat to 60°C)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.4437 mL 32.2186 mL 64.4371 mL
    5 mM 1.2887 mL 6.4437 mL 12.8874 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

    C2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 12.5 mg/mL (80.55 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    COA (259 KB) HNMR (239 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.4437 mL 32.2186 mL 64.4371 mL 161.0929 mL
    5 mM 1.2887 mL 6.4437 mL 12.8874 mL 32.2186 mL
    10 mM 0.6444 mL 3.2219 mL 6.4437 mL 16.1093 mL
    15 mM 0.4296 mL 2.1479 mL 4.2958 mL 10.7395 mL
    20 mM 0.3222 mL 1.6109 mL 3.2219 mL 8.0546 mL
    25 mM 0.2577 mL 1.2887 mL 2.5775 mL 6.4437 mL
    30 mM 0.2148 mL 1.0740 mL 2.1479 mL 5.3698 mL
    40 mM 0.1611 mL 0.8055 mL 1.6109 mL 4.0273 mL
    50 mM 0.1289 mL 0.6444 mL 1.2887 mL 3.2219 mL
    60 mM 0.1074 mL 0.5370 mL 1.0740 mL 2.6849 mL
    80 mM 0.0805 mL 0.4027 mL 0.8055 mL 2.0137 mL
    100 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6109 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    BCH Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BCH20448-79-72-Amino-2-norbornanecarboxylic acid LAT1-IN-1ApoptosisInhibitorinhibitorinhibit

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