1. Apoptosis
  2. Apoptosis
  3. LAT1-IN-1

LAT1-IN-1 (Synonyms: BCH)

Cat. No.: HY-108540
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LAT1-IN-1 (BCH) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis.

For research use only. We do not sell to patients.

LAT1-IN-1 Chemical Structure

LAT1-IN-1 Chemical Structure

CAS No. : 20448-79-7

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Description

LAT1-IN-1 (BCH) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis[1][2][3].

IC50 & Target

LAT1[1]

In Vitro

LAT1-IN-1 (1-100 mM; 3 days; KYSE30 and KYSE150 esophageal cancer cells) treatment suppresses cell proliferation in a dose-dependent manner[1].
LAT1-IN-1 (30 mM; 24 and 48 hours; KYSE30 and KYSE150 cells) treatment significantly increases cell population in the G0/G1 phase in both KYSE30 and KYSE150 cells, indicating that LAT1-IN-1 induces cell cycle arrest at G1 phase[1].
LAT1-IN-1 (30 mM; 0-24 hours; KYSE30 and KYSE150 cells) treatment decreases phosphorylation of 4E-BP1 and p70S6K at 30 minutes and the decrease is continued for 24 hours. The amount of mTOR, 4E-BP1, and p70S6K proteins is slightly decreased[1].

Cell Proliferation Assay[1]

Cell Line: KYSE30 and KYSE150 esophageal cancer cells
Concentration: 1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM
Incubation Time: 3 days
Result: Cell proliferation was suppressed in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: KYSE30 and KYSE150 cells
Concentration: 30 mM
Incubation Time: 24 and 48 hours
Result: Cell cycle arrest.

Western Blot Analysis[1]

Cell Line: KYSE30 and KYSE150 cells
Concentration: 30 mM
Incubation Time: 0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours
Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.
In Vivo

LAT1-IN-1 (200 mg/kg; intravenous injection; daily; for 14 days; male BALB/c nude mice) treatment significantly delays tumor growth and decreases glucose metabolism, indicating that LAT1 inhibition potentially suppresses esophageal cancer growth in vivo[1].

Animal Model: Male BALB/c nude mice (5-week-old) with KYSE150 cells[1]
Dosage: 200 mg/kg
Administration: Intravenous injection; daily; for 14 days
Result: Significantly delayed tumor growth and decreased glucose metabolism.
Molecular Weight

155.19

Formula

C₈H₁₃NO₂

CAS No.

20448-79-7

SMILES

O=C(C1(N)C(C2)CCC2C1)O

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Storage

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