LW-1
LW-1 is a fluorescent probe targeting polarity detection of lipid droplets (LD). LW-1 produces a strong solvatochromic effect via the intramolecular charge transfer (ICT) mechanism: as environmental polarity increases, the emission wavelength red-shifts and the fluorescence intensity decreases significantly (emission at 557 nm in toluene and 642 nm in acetonitrile, with an 85 nm red-shift); fluorescence enhances in low-polarity lipid droplets. LW-1 can be used for lipid droplet polarity detection, differentiation between cancer cells and normal cells, monitoring of lipid droplet polarity changes under different physiological conditions such as starvation, oleic acid treatment and oxidative stress, as well as ex vivo imaging of polarity changes in lung tissues of asthmatic mice. The detection wavelength for cell imaging with LW-1 is Ex/Em = 405/500-550 nm (green channel).
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- CAS. Nr.: 1510814-96-6
- Formel: C26H14N2
- Molecular Weight:354.40
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
In Vitro
Guidelines (The recommended experimental protocol below is for guidance only and should be adjusted according to your specific requirements)
1. Stock Solution Preparation
1.1 Solvent: Most dyes are typically dissolved in organic solvents, such as anhydrous DMSO.
1.2 Concentration Recommendation: It is generally recommended to prepare a high-concentration stock solution of 1-10 mM.
2. Working Solution Preparation
2.1 Diluent: Phosphate buffer (pH 7.4, 10 mM, 5% DMSO).
2.2 Working Concentration: 1-10 μM.
2.3 Notes: Adjust the working solution concentration as needed; prepare the solution immediately before use.
3. Staining Procedure
3.1 Sample Type Description
3.1.1 Adherent cells: HepG2, Siha, Hela, 3T3, 4T1, HL-7702 cells.
3.2 Incubation Conditions
3.2.1 Adherent cells: Incubate at 37°C for 30 min, and light avoidance is recommended during incubation.
3.3 Washing Step: After incubation, wash the cells 2-3 times with pre-warmed PBS or serum-free medium to remove free probes and reduce background fluorescence.
4. Control Setup
4.1 Unstained Control: Used to adjust instrument voltage and eliminate autofluorescence of cells.
5. Detection and Analysis
5.1 Instrument Type: Confocal microscope, fluorescence microscope.
5.2 Excitation/Emission Wavelength: Excitation wavelength is 405 nm (410 nm for spectral experiments), and the emission channel collects signals at 500-550 nm (green channel).
5.3 Result Analysis: LW-1 localizes specifically to lipid droplets, and its fluorescence intensity is negatively correlated with lipid droplet polarity-lower polarity corresponds to stronger fluorescence. Lipid droplets in cancer cells (e.g., HepG2, 4T1) have lower polarity than those in normal cells (e.g., HL-7702, 3T3), so cancer cells exhibit stronger fluorescence; starvation and oleic acid stimulation can reduce lipid droplet polarity (enhance fluorescence), while H2O2 stimulation can increase lipid droplet polarity (weaken fluorescence). Changes in lipid droplet polarity can be analyzed semi-quantitatively based on changes in fluorescence intensity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
1. Solvent
The literature does not specify the solvent for in vivo working solution.
2. Model Establishment and Staining
2.1 Asthma model: A mouse asthma model is established via ovalbumin (OVA) induction, with the successful modeling indicated by symptoms such as sneezing, nose scratching, and tachypnea in mice.
2.2 Treatment group: Asthmatic mice are treated with Ketotifen fumarate (HY-B0157A) nasal drops for 3 days.
2.3 Grouping: Normal group, asthma group, and treatment group.
2.4 Ex vivo tissue staining: After mice are sacrificed, lung tissues are harvested to prepare sections. The sections are immersed in a working solution containing LW-1 (10 μM), incubated at 37°C in the dark for 30 min, washed with PBS, and then imaged.
2.5 Ex vivo organ imaging: After sacrifice, intact lung organs are harvested, immersed in a working solution containing LW-1 and incubated for 2 h, followed directly by fluorescence imaging at the organ level.
3. Detection and Analysis
3.1 Instrument settings: Fluorescence microscopes or animal imagers are recommended. The excitation wavelength is 405 nm, and the emission channel collects signals at 500-550 nm.
3.2 Result analysis: The fluorescence intensity of lung tissues and lung organs in asthmatic mice is significantly higher than that in normal mice, indicating reduced polarity of lipid droplets in lung tissues under asthmatic conditions; after treatment with Ketotifen fumarate, the fluorescence intensity decreases but remains higher than that in the normal group, indicating partial recovery of lung polarity after treatment. The results demonstrate that LW-1 enables the detection of lipid droplet polarity changes at both tissue and organ levels, and can be used in studies on the diagnosis and efficacy evaluation of polarity-related diseases such as asthma.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1510814-96-6
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Molecular Weight 354.40
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Formel C26H14N2
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SMILES
N#C/C(C#N)=C/C1=CC=C(C=C1)C2=C3C=CC4=CC=CC5=CC=C(C3=C45)C=C2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
LW-1 Related Classifications
HY-D3071 Related Classifications
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