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Cat. No.: HY-163275

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MDM2-IN-24

MDM2-IN-24 (compound A3f) exhibits MDM2-inhibiting and MDMX-activating properties in triple-negative breast cancer (TNBC) cells, with apoptotic and anti-proliferative activities.

For research use only. We do not sell to patients.

Formula: C₃₃H₂₅Br₂P
Molecular Weight:612.33

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (279 KB)

MDM2-IN-24 Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

MDM2-IN-24 (compound A3f) exhibits MDM2-inhibiting and MDMX-activating properties in triple-negative breast cancer (TNBC) cells, with apoptotic and anti-proliferative activities^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Breast cancer cell line	IC₅₀	3.7 μM Compound: A3f	Cytotoxicity against human Breast cancer cell line Cytotoxicity against human Breast cancer cell line	[PMID: 39185935]
Breast carcinoma cell	IC₅₀	3.7 μM Compound: A3f	Cytotoxicity against human Triple-negative breast cancer cell Cytotoxicity against human Triple-negative breast cancer cell	[PMID: 39185935]

In Vitro

MDM2-IN-24 is a G-quadruplex ligand, which binds with G-rich sequences of MDM2 and MDMX with K_ds of 4.9 and 5.2 μM, respectively^[1].
MDM2-IN-24 (6 μM) inhibits MDM2 and thereby induces proliferation and apoptosis in TNBC cells^[1].
MDM2-IN-24 (6 μM) exhibits cytotoxicity in HCC1937 cells (survival rate is 27.7%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

HCC1937
Concentration:

3 μM
Incubation Time:
Result:

Decreased mRNA levels of MDM2 and increased mRNA levels of MDMX.

Western Blot Analysis^[1]

Cell Line:

HCC1937
Concentration:

0-6 μM
Incubation Time:

24 h
Result:

Decreased MDM2 expression, increased levels of MDMX and cleaved caspase-3 in normal HCC1937.
Reactivated cleaved caspase-3 in HCC1937 cells with siMDMX.

Chemical Information

Molecular Weight 612.33
Formula C₃₃H₂₅Br₂P
SMILES

BrC1=C2C=CC=CC2=C(C[P+](C3=CC=CC=C3)(C4=CC=CC=C4)C5=CC=CC=C5)C6=CC=CC=C16.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Feng Y, et al., Targeting G-rich sequence to regulate the transcription of murine double minute (MDM) genes in triple-negative breast cancers. Eur J Med Chem. 2024 Jan 25;267:116156. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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