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  3. Fluorescent Dye
  4. Methyl Blue

Methyl blue belongs to the group of triaminotriphenylmethane dyes. Methyl blue is widely used as antiseptic dye in polychrome staining method and has applications in histological and microbiological staining solutions. Methyl blue has been used as a model to study the effect of various catalysts on photodegradation of dyes.

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Methyl Blue Chemical Structure

Methyl Blue Chemical Structure

CAS No. : 28983-56-4

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Based on 1 publication(s) in Google Scholar

Methyl Blue Related Antibodies

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Description

Methyl blue belongs to the group of triaminotriphenylmethane dyes. Methyl blue is widely used as antiseptic dye in polychrome staining method and has applications in histological and microbiological staining solutions. Methyl blue has been used as a model to study the effect of various catalysts on photodegradation of dyes[1][2].

Molecular Weight

799.80

Formula

C37H27N3Na2O9S3
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=S(C1=CC=CC=C1/N=C2C=C/C(C=C\2)=C(C3=CC=C(NC4=CC=CC=C4S(=O)(O)=O)C=C3)\C5=CC=C(NC6=CC=CC=C6S(=O)(O[Na])=O)C=C5)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 20 mg/mL (25.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2503 mL 6.2516 mL 12.5031 mL
5 mM 0.2501 mL 1.2503 mL 2.5006 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 16.67 mg/mL (20.84 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2503 mL 6.2516 mL 12.5031 mL 31.2578 mL
5 mM 0.2501 mL 1.2503 mL 2.5006 mL 6.2516 mL
10 mM 0.1250 mL 0.6252 mL 1.2503 mL 3.1258 mL
15 mM 0.0834 mL 0.4168 mL 0.8335 mL 2.0839 mL
20 mM 0.0625 mL 0.3126 mL 0.6252 mL 1.5629 mL
25 mM 0.0500 mL 0.2501 mL 0.5001 mL 1.2503 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Methyl Blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl Blue28983-56-4Fluorescent DyeInhibitorinhibitorinhibit

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