Methyl Blue
Based on 1 Customer Validation
Methyl blue belongs to the group of triaminotriphenylmethane dyes. Methyl blue is widely used as antiseptic dye in polychrome staining method and has applications in histological and microbiological staining solutions. Methyl blue has been used as a model to study the effect of various catalysts on photodegradation of dyes.
For research use only. We do not sell to patients.
- CAS No.: 28983-56-4
- Formula: C37H27N3Na2O9S3
- Molecular Weight:799.80
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
Biological Activity
Methyl Blue spectrum shows a slight red shift of λmax and an increase of the absorbance in the presence of TX-100[1].
Methyl blue is adsorbed on ZnO nanoparticles, and the capacity reaches 10.70 mmol/g[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 28983-56-4
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Appearance Solid
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Molecular Weight 799.80
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Formula C37H27N3Na2O9S3
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Color Brown to reddish brown
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SMILES
O=S(C1=CC=CC=C1/N=C2C=C/C(C=C\2)=C(C3=CC=C(NC4=CC=CC=C4S(=O)(O)=O)C=C3)\C5=CC=C(NC6=CC=CC=C6S(=O)(O[Na])=O)C=C5)(O[Na])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
H2O : 20 mg/mL (25.01 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2503 mL | 6.2516 mL | 12.5031 mL | 31.2578 mL |
| 5 mM | 0.2501 mL | 1.2503 mL | 2.5006 mL | 6.2516 mL | |
| 10 mM | 0.1250 mL | 0.6252 mL | 1.2503 mL | 3.1258 mL | |
| 15 mM | 0.0834 mL | 0.4168 mL | 0.8335 mL | 2.0839 mL | |
| 20 mM | 0.0625 mL | 0.3126 mL | 0.6252 mL | 1.5629 mL | |
| 25 mM | 0.0500 mL | 0.2501 mL | 0.5001 mL | 1.2503 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.