Methylene blue indicator
Based on 1 publication(s) in Google Scholar
Methylene blue indicator is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
For research use only. We do not sell to patients.
- Purity: 99.3%
- CAS No.: 7220-79-3
- Formula: C16H24ClN3O3S
- Molecular Weight:373.90
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Methylene blue indicator
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Biological Activity
Methylene blue indicator is widely employed as a redox indicator, exhibiting a blue color in its oxidized state and becoming colorless upon reduction[1].
Methylene blue indicator is commonly used for cell staining to visualize cell morphology, which primarily stains the nucleus due to its strong affinity for nucleic acids (DNA and RNA)[2].
Methylene blue indicator is also used for bacterial staining and capsule stainng[3].
Methylene blue indicator can be used as an indicator to recognize the single base mismatch in duplex DNA[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7220-79-3
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Appearance Solid
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Molecular Weight 373.90
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Formula C16H24ClN3O3S
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Color Green to black
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SMILES
CN(C1=CC(SC2=C/C(C=CC2=N3)=[N+](C)\C)=C3C=C1)C.[3 H2O].[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Enhancing radiotherapy in triple-negative breast cancer with hesperetin-induced ferroptosis via AURKA targeting nanocomposites. [Abstract]2024 Nov 30;22(1):744. PMID: 39614277
Solvent & Solubility
DMSO : 6.67 mg/mL (17.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
References
[1]. Farjami E, et al. DNA interactions with a Methylene Blue redox indicator depend on the DNA length and are sequence specific. Analyst. 2010 Jun;135(6):1443-8. [Content Brief]
[3]. Breakwell DP, et al. Differential staining of bacteria: capsule stain. Curr Protoc Microbiol. 2009 Nov;Appendix 3:Appendix 3I. [Content Brief]
[4]. Zhang Z, et al. Distinction of single base mismatches in duplex DNA using methylene blue as optical indicator. Analyst. 2010 Nov;135(11):2960-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6745 mL | 13.3726 mL | 26.7451 mL | 66.8628 mL |
| 5 mM | 0.5349 mL | 2.6745 mL | 5.3490 mL | 13.3726 mL | |
| 10 mM | 0.2675 mL | 1.3373 mL | 2.6745 mL | 6.6863 mL | |
| 15 mM | 0.1783 mL | 0.8915 mL | 1.7830 mL | 4.4575 mL |
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