N,N'-Dibenzylethylenediamine
Based on 1 Customer Validation
N,N'-Dibenzylethylenediamine forms chelator with metals. N,N'-Dibenzylethylenediamine interacts with penicillin, forms a stable and tasteless salt of penicillin.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 140-28-3
- Formula: C16H20N2
- Molecular Weight:240.34
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>50 μM
Compound: 11
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Cytotoxicity against human HuH7 cells
Cytotoxicity against human HuH7 cells
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[PMID: 24177358] |
| Huh-7 | EC50 |
>50 μM
Compound: 11
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Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy
Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy
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[PMID: 24177358] |
Chemical Information
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CAS No. 140-28-3
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Appearance Liquid (Density: 1.024 g/cm3)
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Molecular Weight 240.34
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Formula C16H20N2
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Color Colorless to light yellow
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SMILES
C1(CNCCNCC2=CC=CC=C2)=CC=CC=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 200 mg/mL (832.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (20.80 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1608 mL | 20.8039 mL | 41.6077 mL | 104.0193 mL |
| 5 mM | 0.8322 mL | 4.1608 mL | 8.3215 mL | 20.8039 mL | |
| 10 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL | 10.4019 mL | |
| 15 mM | 0.2774 mL | 1.3869 mL | 2.7738 mL | 6.9346 mL | |
| 20 mM | 0.2080 mL | 1.0402 mL | 2.0804 mL | 5.2010 mL | |
| 25 mM | 0.1664 mL | 0.8322 mL | 1.6643 mL | 4.1608 mL | |
| 30 mM | 0.1387 mL | 0.6935 mL | 1.3869 mL | 3.4673 mL | |
| 40 mM | 0.1040 mL | 0.5201 mL | 1.0402 mL | 2.6005 mL | |
| 50 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
| 60 mM | 0.0693 mL | 0.3467 mL | 0.6935 mL | 1.7337 mL | |
| 80 mM | 0.0520 mL | 0.2600 mL | 0.5201 mL | 1.3002 mL | |
| 100 mM | 0.0416 mL | 0.2080 mL | 0.4161 mL | 1.0402 mL |
N,N'-Dibenzylethylenediamine Related Classifications
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