N3-Aca-Aca-OH
N3-Aca-Aca-OH is a bifunctional linker containing azide groups, which is a bifunctional diaminohexanoic acid (Aca dimer) and is mainly used for click chemistry coupling, ADC linker construction, and PROTAC linker design.
For research use only. We do not sell to patients.
- CAS No.: 866363-71-5
- Formula: C12H22N4O3
- Molecular Weight:270.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
N3-Aca-Aca-OH contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo ring tension-promoted acrylonitrile-azide cycloaddition reaction (SPAAC) with molecules containing DBCO or BCN groups.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 866363-71-5
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Molecular Weight 270.33
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Formula C12H22N4O3
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SMILES
[N-]=[N+]=NCCCCCC(NCCCCCC(O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
N3-Aca-Aca-OH Related Classifications
HY-151861 Related Classifications
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