Neuro-DiO 4-chlorobenzenesulfonate 

Neuro-DiO 4-chlorobenzenesulfonate is a hydrophobic C₁₈ alkyl chain carbocyanine dye with green fluorescence, commonly used as a vascular marker, cell internalizer and deposition agent. Neuro-DiO 4-chlorobenzenesulfonate inserts its alkyl chain into the endothelial plasma membrane via liposome-mediated perfusion to achieve vascular labeling. Neuro-DiO chlorobenzenesulfonate can also stain the cell membrane and cytoplasm of cancer cells to assist in confocal microscopy observations. Neuro-DiO chlorobenzenesulfonate can be released from nanosponges and accumulate on the surface of mouse retina, then internalize into retinal ganglion cells, which is applicable to researches related to glaucoma and other diseases. It should be noted that during liposome-mediated vascular staining in mice, Neuro-DiO 4-chlorobenzenesulfonate may cause leakage of airway lavage fluid^[1][2][3].

Neuro-DiO 4-chlorobenzenesulfonate (5 μg/mL) effectively stains the membranes and cytoplasm of C6 rat brain glioma cells and MCF-7 human breast adenocarcinoma cells for use as a counterstain in confocal microscopy studies of lipid nanoparticle localization^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Neuro-DiO 4-chlorobenzenesulfonate (1 μL of 50 nm nanosponges loaded with Neuro-DiO; intravitreal injection; both eyes) leads to a time-dependent increase in retinal deposition of Neuro-DiO (reaching up to 71.4% retinal surface coverage by 28 days post-injection) and internalization of the dye by retinal ganglion cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1086.08

C₆₇H₁₀₅ClN₂O₅S

CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2N(CCCCCCCCCCCCCCCCCC)C(C=C(C(C)(C)C)C=C3)=C3O/2)OC4=C1C=C(C(C)(C)C)C=C4.ClC5=CC=C(S(=O)([O-])=O)C=C5

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Konno A, et al. Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures. Sci Rep. 2020;10(1):12613. Published 2020 Jul 28.  [Content Brief]

  [2]. Mishchenko E V, et al. Nanoemulsions and solid lipid nanoparticles with encapsulated doxorubicin and thymoquinone[J]. Colloid Journal, 2023, 85(5): 736-745.

  [3]. Lambert WS, et al. Nanosponge-Mediated Drug Delivery Lowers Intraocular Pressure. Transl Vis Sci Technol. 2015;4(1):1. Published 2015 Jan 13.  [Content Brief]