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Cat. No.: HY-134821

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NHE-1-IN-1

NHE-1-IN-1 is a non-acylguanidine NHE-1 inhibitor with IC₅₀ values of 0.0065 μM and 9 μM for human NHE-1 and NHE-2, respectively. NHE-1-IN-1 inhibits NHE-1 function by suppressing NHE-1-mediated Na⁺-dependent intracellular pH recovery after acidification. NHE-1-IN-1 can be used for research on NHE-1-related cardiovascular regulation.

For research use only. We do not sell to patients.

CAS No.: 335063-97-3
Formula: C₂₁H₂₄FN₅O
Molecular Weight:381.46

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

NHE-1-IN-1 Chemical Structure

CAS No.: 335063-97-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

NHE-1

0.0065 μM (IC₅₀)

NHE-2

9 μM (IC₅₀)

In Vitro

NHE-1-IN-1 (compound 9t) inhibits sodium-dependent pH recovery after acidification in AP1 cells expressing human NHE-1, with an IC₅₀ of 0.0065 μM^[1].
NHE-1-IN-1 inhibits NHE-2 activity in AP1 cells expressing human NHE-2 with an IC₅₀ of 9 μM and exhibits 1400-fold selectivity over NHE-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 335063-97-3
Molecular Weight 381.46
Formula C₂₁H₂₄FN₅O
SMILES

FC1=CC=C(C=C1C)C=2C=NC(=NC2N3CCC(C=4NC=NC4C)CC3)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Atwal KS, et al. Synthesis and biological activity of 5-aryl-4-(4-(5-methyl-1H-imidazol-4-yl)piperidin-1-yl)pyrimidine analogs as potent, highly selective, and orally bioavailable NHE-1 inhibitors. Bioorganic & medicinal chemistry letters. 2006 Sep 15;16(18):4796-9.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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