NIR-βgal-2

NIR-βgal-2 displays superior sensitivity (detection limit = 2.0 × 10^-3 U/mL), great affinity (K_m = 1.84 μM), and catalytic efficiency (k_cat/K_m = 0.24 μM^-1 s^-1) for β-galactosidase^[1].
The survival rate of cells (SKOV-3, 4T1, A549, and L02 cells) in the presence of NIR-βgal-2 is higher than 80% after 24 h, suggesting that the probe had low cytotoxicity toward the cells^[1].
Human ovarian carcinoma cells (SKOV-3 cells) and mouse breast cancer cells (4T1 cells) that endogenously overexpress β-galactosidase are chosen as cell models for this study. In comparison, human normal liver cells (L02 cells) and human lung cancer cells (A549 cells) are selected as negative groups. After incubating with NIR-βgal-2 (5 μM) for 30 min, can observe bright fluorescence signals in SKOV-3 and 4T1 cells, whereas faint NIR fluorescence is observed in A549 and L02 cells. NIR-βgal-2 could differentiate efficiently and rapidly detect β-galactosidase in different cells and can distinguish cancer cells that overexpress β-galactosidase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.