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Cat. No.: HY-126473

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NE97220

NE97220 is a biochemical reagent. NE97220 induces macrophage apoptosis and reduces their viability, leading to internucleosomal DNA fragmentation. NE97220 inhibits the growth of Trypanosoma brucei rhodesiense. NE97220 is used in research on human African trypanosomiasis and immune-related studies.

For research use only. We do not sell to patients.

CAS No.: 70010-76-3
Formula: C₇H₁₂N₂O₆P₂
Molecular Weight:282.13

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

NE97220 Chemical Structure

CAS No.: 70010-76-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
J774	EC50	62 μM	Reduction of viability in murine macrophage-like J774 cells assessed by MTT assay after 48 hrs incubation. Reduction of viability in murine macrophage-like J774 cells assessed by MTT assay after 48 hrs incubation.	9797474

In Vitro

NE97220 (48 h) reduces J774 cell viability with an EC₅₀ of 62 μM^[1].
NE97220 (100 μM; 48 h) causes internucleosomal DNA fragmentation in J774 macrophages^[1].
NE97220 (100 μM; 24 h) inhibits protein prenylation in J774 macrophages^[1].
NE97220 (100 μM; 48 h) induces apoptosis in J774 macrophages, resulting in 31% apoptotic cells at 100 μM^[1].
NE97220 (30 μM; 3-24 h) induces IPP/ApppI accumulation in MCF-7 cells after both continuous and pulse exposure, and is among the compounds with lower K_isom values that induce fast IPP/ApppI production^[2].
NE97220 (0.7 μM) potently inhibits the in vitro growth of Trypanosoma brucei rhodesiense, with an IC₅₀ of 0.7 μM^[3].
NE97220 inhibits GGPPSase activity with an IC₅₀ of 220.0 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

J774
Concentration:

100 μM
Incubation Time:

48 h
Result:

Caused an increase in the proportion of apoptotic cells, with a percentage apoptosis of 31%.

In Vivo

NE97220 (0.001 mg P/kg) inhibits bone resorption in the growing rat model with an LED of 0.001 mg P/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 70010-76-3
Molecular Weight 282.13
Formula C₇H₁₂N₂O₆P₂
SMILES

O=P(O)(C(P(O)(O)=O)NC1=NC=CC=C1C)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Luckman SP, et al. Heterocycle-containing bisphosphonates cause apoptosis and inhibit bone resorption by preventing protein prenylation: evidence from structure-activity relationships in J774 macrophages. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 1998 Nov;13(11):1668-78.

[2]. Räikkönen J, et al. Correlation between time-dependent inhibition of human farnesyl pyrophosphate synthase and blockade of mevalonate pathway by nitrogen-containing bisphosphonates in cultured cells. Biochemical and biophysical research communications. 2011 Apr 22;407(4):663-7.

[3]. Xie A, et al. Bisphosphonates. Internet Electronic Journal of Molecular Design. 2004;3:622-50.

[4]. Ebrahimpour A, et al. Determination of solubility and calcium ion stability constants of a phosphonoalkylphosphinate (PAP) and bisphosphonates (BPs) such as EHDP, Risedronate, Alendronate, 3-pic AMBP, and 3-pic AMPMP. InMineral scale formation and inhibition 1995 (pp. 295-305). Boston, MA: Springer US.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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