PAB-QMSF2-PEG2-Biotin
PAB-QMSF2-PEG2-Biotin is a biotin probe for the CAT-S photocatalytic proximity labeling system. PAB-QMSF2-PEG2-Biotin undergoes uncaging under the action of a mitochondria-targeted iridium photocatalyst and blue light (450 nm) to release difluorothioquinone methide (thioQM), which covalently labels nucleophilic residues (Lys/Tyr/Glu/Asp/Trp, etc.) of neighboring proteins. PAB-QMSF2-PEG2-Biotin preferentially labels mitochondrial matrix proteins in live cells. PAB-QMSF2-PEG2-Biotin is conjugated with a biotin tag, enabling detection or isolation of captured proteins. PAB-QMSF2-PEG2-Biotin can be used for research related to type 2 diabetes.
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- Formule: C31H39F2N7O5S2
- Masse moléculaire:691.81
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Description
In Vitro
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) produced high photocatalytic protein labeling efficiency among QM/thioQM probes tested in live HeLa cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min)-mediated CAT-S labeling produced mitochondria-localized biotinylation signals in live HeLa and HEK293T cells, with a high degree of colocalization[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min), when used in combination with CAT-S, captured the mitochondrial proteome comprising 326 known mitochondrial proteins with 83% specificity in live HeLa cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captured 334 known mitochondrial proteins with 79% specificity in live HEK293T cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captured 336 known mitochondrial proteins with 87% specificity in live K562 cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min), when used in combination with CAT-S, captured mitochondrial proteomes containing 261 and 228 known mitochondrial proteins in dissociated mouse kidney and spleen primary cells, respectively[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captured 219 known mitochondrial proteins with 62% specificity in human primary T cells from PBMCs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:SF2 100 μM
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Incubation Time:12 min (blue LED ~4 mW/cm2); 10 min (dark)
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Result:All four probes (OF1, OF2, SF1, and SF2) showed significant protein labeling in the presence of Ir1.
Displayed the highest labeling efficiency.
Arylazide probe AzPh showed nearly no labeling.
Ir1 displayed much higher labeling activity than the other Ir catalysts tested.
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Cell Line:HeLa and HEK293T cells
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Concentration:SF2 100 μM
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Incubation Time:12 min (blue LED ~4 mW/cm2)
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Result:Biotinylation signal precisely colocalized with the mitochondria marker.
Pearson’s R values were 0.87 (HeLa) and 0.90 (HEK293T).
Chemical Information
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Masse moléculaire 691.81
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Formule C31H39F2N7O5S2
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SMILES
[H][C@]12CS[C@@H](CCCCC(NCCOCCOCCNC(C3=CC=C(SCC4=CC=C(N=[N+]=[N-])C=C4)C(C(F)F)=C3)=O)=O)[C@]1(NC(N2)=O)[H]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PAB-QMSF2-PEG2-Biotin Related Classifications
HY-186341 Related Classifications
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