PAB-QMSF2-PEG2-Biotin
PAB-QMSF2-PEG2-Biotin is a biotin probe for the CAT-S photocatalytic proximity labeling system. PAB-QMSF2-PEG2-Biotin undergoes uncaging under the action of a mitochondria-targeted iridium photocatalyst and blue light (450 nm) to release difluorothioquinone methide (thioQM), which covalently labels nucleophilic residues (Lys/Tyr/Glu/Asp/Trp, etc.) of neighboring proteins. PAB-QMSF2-PEG2-Biotin preferentially labels mitochondrial matrix proteins in live cells. PAB-QMSF2-PEG2-Biotin is conjugated with a biotin tag, enabling detection or isolation of captured proteins. PAB-QMSF2-PEG2-Biotin can be used for research related to type 2 diabetes.
For research use only. We do not sell to patients.
- Formula: C31H39F2N7O5S2
- Molecular Weight:691.81
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
PAB-QMSF2-PEG2-Biotin (SF2) (100 μM; 5 min) is a thioQM probe used for photocatalytic BSA labeling, producing high labeling intensity and surface-exposed, Lys-preferring thioQM modifications[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) produces high photocatalytic protein labeling efficiency among QM/thioQM probes tested in live HeLa cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min)-mediated CAT-S labeling produces mitochondria-localized biotinylation signals in live HeLa and HEK293T cells, with a high degree of colocalization[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min), when used in combination with CAT-S, captures the mitochondrial proteome comprising 326 known mitochondrial proteins with 83% specificity in live HeLa cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captures 334 known mitochondrial proteins with 79% specificity in live HEK293T cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captures 336 known mitochondrial proteins with 87% specificity in live K562 cells[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min), when used in combination with CAT-S, captures mitochondrial proteomes containing 261 and 228 known mitochondrial proteins in dissociated mouse kidney and spleen primary cells, respectively[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S captures 219 known mitochondrial proteins with 62% specificity in human primary T cells from PBMCs[1].
PAB-QMSF2-PEG2-Biotin (100 μM; 12 min) combined with CAT-S quantifies mitochondrial proteome changes in primary cells from isolated diabetic mouse kidney, including Cpt1b upregulation and Aldh3a2/Acsm2 downregulation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HeLa cells
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Concentration:SF2 100 μM
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Incubation Time:12 min (blue LED ~4 mW/cm2); 10 min (dark)
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Result:All four probes (OF1, OF2, SF1, and SF2) showed significant protein labeling in the presence of Ir1.
Displayed the highest labeling efficiency.
Arylazide probe AzPh showed nearly no labeling.
Ir1 displayed much higher labeling activity than the other Ir catalysts tested.
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Cell Line:HeLa and HEK293T cells
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Concentration:SF2 100 μM
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Incubation Time:12 min (blue LED ~4 mW/cm2)
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Result:Biotinylation signal precisely colocalized with the mitochondria marker.
Pearson’s R values were 0.87 (HeLa) and 0.90 (HEK293T).
Chemical Information
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Molecular Weight 691.81
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Formula C31H39F2N7O5S2
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SMILES
[H][C@]12CS[C@@H](CCCCC(NCCOCCOCCNC(C3=CC=C(SCC4=CC=C(N=[N+]=[N-])C=C4)C(C(F)F)=C3)=O)=O)[C@]1(NC(N2)=O)[H]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PAB-QMSF2-PEG2-Biotin Related Classifications
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