country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-175518

Copy Link Print Information

PADI4-IN-1

PADI4-IN-1 is a potent peptidylarginine deiminase isoform 4 (PADI4) inhibitor with an IC₅₀ of 1.5 μM and SI (PADI1/PADI4) of 52.1. PADI4-IN-1 can inhibit cellular citrullination events. PADI4-IN-1 can be used for the research of inflammation, such as rheumatoid arthritis.

For research use only. We do not sell to patients.

Formula: C₂₁H₂₃NO₃
Molecular Weight:337.41

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

PADI4-IN-1 Chemical Structure

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PADI4

1.5 μM (IC₅₀)

In Vitro

PADI4-IN-1 (Compound 7) (10-100 μM, 50 mins) decreases total cellular citrullination events and histone H3 citrullination mediated by PADI4 in HL-60 cell lysates^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HL-60 cells
Concentration:

10 and 100 μM
Incubation Time:

50 mins
Result:

Decreased total HL-60 cellular citrullination events and histone H3 citrullination mediated by PADI4.

In Vivo

PADI4-IN-1 (Compound 7) (20 mg/kg, i.p., at days 3, 4, 5, 6, and 7) ameliorates the severity and pathologic progress in collagen type II antibody/LPS (HY-D1056) induced rheumatoid arthritis mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Collagen type II antibody/LPS (HY-D1056) induced rheumatoid arthritis mice models^[1]
Dosage:

20 mg/kg
Administration:

Intraperitoneally injection
Result:

Decreased the arthritis score of the mice by 66 % treated at days 4, 5, 6, and 7.
Decreased the arthritis score of the mice by 100 % treated at days 3 and 4.
Reduced the expression of IL-6, IL-1β, IFN-γ, RANKL, IL-17A, CXCL15, ANXA2, HIF-1α, iNOS, and TNF-αtreated at days 3 and 4.
Reduced inflammatory cell influx, pannus formation, cartilage destruction, cartilage remnant, edema, and accumulation of inflammatory cells (mononuclear cells and neutrophil).
Reduced the erosion of cartilage and bone.
Reduced the levels of citrullinated antigens.

Chemical Information

Molecular Weight 337.41
Formula C₂₁H₂₃NO₃
SMILES

COC1=CC2=C(C(OC)=C1)OC3(N(C4=CC=CC=C4C3(C)C)C)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yang CW, et al. Synthesis and evaluation of a novel class of spiro[chromene-2,2'-indoline] derivatives as potent inhibitors of peptidylarginine deiminase IV to treat rheumatoid arthritis. Eur J Med Chem. 2025 Nov 15;298:117985. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

Get Quote In-stock