Pcna Rat Pre-designed siRNA Set A
Pcna Rat Pre-designed siRNA Set A contains three designed siRNAs for Pcna gene (Rat), as well as a negative control, a positive control, and a FAM-labeled negative control.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Pcna Rat Pre-designed siRNA Set A contains three designed siRNAs for Pcna gene (Rat), as well as a negative control, a positive control, and a FAM-labeled negative control.
Components
Pcna siRNA-1: 5 nmol (HPLC)
Pcna siRNA-2: 5 nmol (HPLC)
Pcna siRNA-3: 5 nmol (HPLC)
siRNA Negative Control: 5 nmol (HPLC)
FAM-labeled siRNA Negative Control: 5 nmol (HPLC)
GAPDH siRNA Positive Control: 5 nmol (HPLC)
Gene ID
Gene Information
Pcna - proliferating cell nuclear antigen Gene (Rat)
Also Known as
PCNAR; Pcna/cyclin
Chemical Information
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SMILES
[Pcna Rat Pre-designed siRNA Set A]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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PCNA Immunodetection Proliferation Assay
PCNA immunodetection measures proliferative activity by detecting proliferating cell nuclear antigen, a nuclear protein associated with DNA polymerase δ function and DNA replication. The assay readout is the proportion of PCNA-positive nuclei among total counted cells, but PCNA labeling is not identical to BrdU labeling because PCNA can mark late G1/early S-associated replication competence and may persist beyond active DNA synthesis depending on fixation and extraction conditions.
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RNA interference technology
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing gene transcription or activating RNA degradation. This mechanism was discovered in plants in 1998 by Andrew Fire and Craig Mello. Today, this phenomenon can be observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)