PNGase F (Immobilized, Microspin)
Based on 2 publication(s) in Google Scholar
PNGase F (Immobilized, Microspin) is a resin in which PNGase F (peptide N-glycosidase F) is covalently coupled to agarose beads, and it is used to remove N-glycans from antibodies, fusion proteins and other N-glycosylated proteins. The enzyme is recombinantly expressed in Escherichia coli, with its sequence derived from Flavobacterium meningsepticum.
For research use only. We do not sell to patients.
- CAS No.: 83534-39-8
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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HY-P2929
PNGase F
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HY-P2929A
PNGase F-Fast
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HY-P2929C
PNGase F (Lyophilized)
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HY-P2929D
PNGase F (MS grade)
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HY-17020
Miglustat
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HY-P2840
Dextranase, Trichoderma reesei
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HY-132177B
α1-2,4,6 Fucosidase O, Omnitrophica bacterium
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HY-132177
α-L-Fucosidase, Microorganism
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HY-132177C
α1-3,4 Fucosidase, Bifidobacterium bifidum
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HY-132177A
α-1-2 Fucosidase, bifidobacterium longum
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HY-P2872
Thioglucosidase
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HY-17020R
Miglustat (Standard)
Publications Citing Use of MedChemExpress (MCE) PNGase F (Immobilized, Microspin)
More
Biological Activity
PNGase F (Immobilized, Microspin) cleaves N-glycans from N-linked glycopeptides, exposes a detectable mark at former glycosylation sites, and enables glycoproteome analysis.PNGase F (Immobilized, Microspin) exhibits reduced efficiency when cleaving N-glycans from hydrazide bead-immobilized glycopeptides due to steric hindrance[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 83534-39-8
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[PNGase F (Immobilized, Microspin)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021;31(1):2-7. [Content Brief]
[2]. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015;87(20):10199-10204. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PNGase F (Immobilized, Microspin) Related Classifications
HY-P2929B Related Classifications
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