Pronase E (Activity ≥ 4000 U/mg)
Based on 27 publication(s) in Google Scholar
Pronase E (Activity ≥ 4000 U/mg) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids.
For research use only. We do not sell to patients.
- CAS No.: 9036-06-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Pronase E (Activity ≥ 4000 U/mg)
More- Cell. 2026 Mar 5;189(5):1451-1464.e27. [Abstract]
- Cancer Res. 2026 Jul 15;86(14):3436-3458. [Abstract]
- Acta Pharm Sin B. 2025 Mar;15(3):1514-1534. [Abstract]
- Pharmacol Res. 2024 Apr:202:107128. [Abstract]
- Phytomedicine. 2026 Jul:156:158180. [Abstract]
- Phytomedicine. 2026 Jul:156:158202. [Abstract]
- Phytomedicine. 2026 Jun:155:158086. [Abstract]
- Phytomedicine. 2025 Dec:149:157524. [Abstract]
- Phytomedicine. 2025 Sep:145:156915. [Abstract]
- Phytomedicine. 2024 Dec:135:156011. [Abstract]
- J Pharm Anal. 2025 Dec;15(12):101307. [Abstract]
- Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. [Abstract]
- Arch Pharm Res. 2026 Mar;49(3):357-374. [Abstract]
- Free Radic Biol Med. 2025 May:232:353-366. [Abstract]
- Chin Med. 2022 Sep 29;17(1):115. [Abstract]
- Biochem Pharmacol. 2025 Nov:241:117158. [Abstract]
- Biochem Pharmacol. 2025 Sep 21;242(Pt 3):117360. [Abstract]
- Food Funct. 2026 Jun 22;17(12):5378-5393. [Abstract]
- Eur J Pharmacol. 2025 Nov 15:1007:178274. [Abstract]
- Int Immunopharmacol. 2026 May 1:176:116462. [Abstract]
- Int Immunopharmacol. 2026 Mar 15:173:116255. [Abstract]
- Am J Respir Cell Mol Biol. 2026 Jan 23:aanaf010. [Abstract]
- Bioorg Chem. 2025 Sep 17:165:109010. [Abstract]
- Virus Res. 2025 May:355:199561. [Abstract]
- G3 (Bethesda). 2022 May 6;12(5):jkac085. [Abstract]
- Res Sq. 2026 Jun 12.
- SSRN. 2025 Feb 4.
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Biological Activity
Description
In Vitro
Pronase E significantly increases the amount of most of the amino acids analysed (PE vs C) , especially Ile, His and Thr[1]. Pronase E Usage Steps:. 1. Preparation of Stock Solution: (1) Dissolve Pronase E in deionized water to prepare a stock solution at a concentration of 5-20 mg/ml. Note: If used for DNA or RNA isolation steps, it is recommended to first heat the solution at 56°C for 15 minutes. (2) Incubate at 37°C for 1 hour. Aliquot the prepared stock solution into single-use samples and store at -20°C; typically stable for one year. 2. DNA Isolation: Directly add Pronase E to the DNA preparation system. The system should contain 0.5-1% SDS to disrupt DNA-protein interactions. Note: Use at a concentration of 250-500 μg protein/ml. Incubation conditions: Incubate at 37°C for 1-4 hours. 3. Protein Digestion: (1) Dissolve approximately 0.2 μM protein in 0.2 ml of 50 mM ammonium bicarbonate buffer (pH 8.0) or phosphate buffer (pH 7.0). (2) Add 1% (w/w) Pronase E. Incubate at 37°C for 24 hours to perform protein digestion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9036-06-0
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Pronase E (Activity ≥ 4000 U/mg)]
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Synonyms
Pronase (Activity ≥ 4000 U/mg)
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Structure Classification
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Initial Source
Streptomyces griseus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (27)
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Journal Impact Factor
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Most Recent
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Cell
Structural decoding of reversible covalent linkage of odorants in human olfactory receptor OR6A2. [Abstract]2026 Mar 5;189(5):1451-1464.e27. PMID: 41570822 -
Cancer Res
TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma. [Abstract]2026 Jul 15;86(14):3436-3458. PMID: 41974002 -
Acta Pharm Sin B
Dihydromyricetin mitigates abdominal aortic aneurysm via transcriptional and post-transcriptional regulation of heme oxygenase-1 in vascular smooth muscle cells. [Abstract]2025 Mar;15(3):1514-1534. PMID: 40370559
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Mar;15(3):1514-1534. [Abstract]
The cell lysis was treated with DHM at concentrations of 0, 5, and 20 mmol/L, and then underwent Pronase E (20 mg/mL, 20 min) digestion.
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Pharmacol Res
Vanillic acid restores homeostasis of intestinal epithelium in colitis through inhibiting CA9/STIM1-mediated ferroptosis. [Abstract]2024 Apr:202:107128. PMID: 38438089
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Apr:202:107128. [Abstract]
Cell lysis was incubated with VA (0, 12.5, 25, and 50 μM) and then subjected to Pronase E (20 μg/mL, 30 min) digestion.
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Phytomedicine
Daphnoretin targeted binding to HSP90AA1 to promote P53 UFMylation and stability thereby inducing apoptosis in colorectal cancer. [Abstract]2026 Jul:156:158180. PMID: 42034025 -
Phytomedicine
Tubeimoside Ⅰ targets sodium/potassium ATPase alpha 1 subunit to selectively eliminate senescent cells and alleviate aging-associated abnormalities. [Abstract]2026 Jul:156:158202. PMID: 42035526 -
Phytomedicine
Usenamine a potentiates anti-CRC activity of sorafenib by inducing autophagy and inhibiting YAP pathway through targeting SOD2. [Abstract]2026 Jun:155:158086. PMID: 41861687 -
Phytomedicine
Icariin rescues immune dysfunction in sepsis through RSK2-driven YAP-cGAS-IFN-β signaling activation. [Abstract]2025 Dec:149:157524. PMID: 41240541
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157524. [Abstract]
Pronase E (20 μg/mL, 30 min). The experimental results of DARST and quantification graphs.
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Phytomedicine
Emodin-induced ERα degradation via SYVN1 alleviates vascular calcification by preventing HIF-1α deacetylation in chronic kidney disease. [Abstract]2025 Sep:145:156915. PMID: 40541127
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:156915. [Abstract]
T/G-HA-VSMC lysates were incubated with Emodin (0, 5, 10, and 20 μM) and then subjected to Pronase E (40 ng/mL, 15 min) digestion. The protein levels of SYVN1 were measured by Western Blot.
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Phytomedicine
Corilagin alleviated intestinal ischemia-reperfusion injury by modulating endoplasmic reticulum stress via bonding with Bip. [Abstract]2024 Dec:135:156011. PMID: 39265205 -
J Pharm Anal
1,8-Cineole ameliorates vascular endothelial senescence in diabetes mellitus by directly targeting and deubiquitinating PPAR-γ in vivo and in vitro. [Abstract]2025 Dec;15(12):101307. PMID: 41492461
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec;15(12):101307. [Abstract]
Western blotting results of the DARTS experiment indicating that the PPAR-g protein was more tolerant to Pronase E (10 mg/mL, 10 min) after 1,8-cineole treatment.
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Cell Chem Biol
2022 Mar 17;29(3):373-385.e6. PMID: 34706270 -
Arch Pharm Res
Homoharringtonine inhibits growth and migration in non-small cell lung cancer via PDIA4-mediated modulation of autophagy and EMT. [Abstract]2026 Mar;49(3):357-374. PMID: 41826608 -
Free Radic Biol Med
Kirenol alleviates cerebral ischemia-reperfusion injury by reducing oxidative stress and ameliorating mitochondrial dysfunction via activating the CK2/AKT pathway. [Abstract]2025 May:232:353-366. PMID: 40090600 -
Chin Med
Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. [Abstract]2022 Sep 29;17(1):115. PMID: 36175965 -
Biochem Pharmacol
Promoting ubiquitin-dependent Drp1 degradation contributes to the protective effect of Astragalin against diabetic renal fibrosis. [Abstract]2025 Nov:241:117158. PMID: 40659133 -
Biochem Pharmacol
Chemoproteomics-based profiling reveals the targeting molecular mechanism of reversal of cisplatin resistance by baicalin in head and neck squamous cell carcinoma. [Abstract]2025 Sep 21;242(Pt 3):117360. PMID: 40987408 -
Food Funct
Cynarin alleviates apical periodontitis by inhibiting macrophage M1 polarization via the PGC1α/ROS signaling axis. [Abstract]2026 Jun 22;17(12):5378-5393. PMID: 42132631 -
Eur J Pharmacol
Neoprzewaquinone A suppresses hepatocellular carcinoma through promoting the ubiquitin-related degradation of EGFR and inhibiting PI3K-AKT pathway. [Abstract]2025 Nov 15:1007:178274. PMID: 41130376 -
Int Immunopharmacol
2026 May 1:176:116462. PMID: 41797086 -
Int Immunopharmacol
Neogambogic acid promotes M-MDSC differentiation into M1 macrophages to inhibit peritoneal metastasis of colorectal cancer. [Abstract]2026 Mar 15:173:116255. PMID: 41579798 -
Am J Respir Cell Mol Biol
Lactylation-YTHDF1 Axis Promotes DNAH5-dependent Ciliary Defense in Pseudomonas aeruginosa Infection. [Abstract]2026 Jan 23:aanaf010. PMID: 41738162 -
Bioorg Chem
Usenamine A, an RGS2 inhibitor, exerts anti-NSCLC activity and enhances cytotoxicity of gemcitabine by inducing ER stress and Notch1-mediated autophagy. [Abstract]2025 Sep 17:165:109010. PMID: 40974657 -
Virus Res
Regulation of Inflammatory Cytokines and Activation of PI3K/Akt Pathway by Yiqi Jiedu Formula in HSV-1-Induced Keratitis: Experimental and Network Pharmacology Evidence. [Abstract]2025 May:355:199561. PMID: 40120648 -
G3 (Bethesda)
Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. [Abstract]2022 May 6;12(5):jkac085. PMID: 35404452 -
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Solvent & Solubility
In Vitro:
H2O : ≥ 66.67 mg/mL
* "≥" means soluble, but saturation unknown.
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL; Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)