A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes

  • FEBS Lett. 2000 Jun 2;474(2-3):137-40. doi: 10.1016/s0014-5793(00)01587-8.
Y Okimotoa  1 ,  A Watanabea ,  E Nikia ,  T Yamashitab ,  N Noguchia
Affiliations
  • 1. Research Center for Advanced Science and Technology, University of Tokyo, Meguro, Japan.
PMID: 10838073 DOI: 10.1016/s0014-5793(00)01587-8
Abstract

Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.

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