Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A

  • Bioorg Med Chem Lett. 2001 Sep 17;11(18):2545-8. doi: 10.1016/s0960-894x(01)00494-2.
N J Kershaw  1 ,  M Mukherji ,  C H MacKinnon ,  T D Claridge ,  B Odell ,  A S Wierzbicki ,  M D Lloyd ,  C J Schofield
Affiliations
  • 1. The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, South Parks Road, OX1 3QY, Oxford, UK.
PMID: 11549466 DOI: 10.1016/s0960-894x(01)00494-2
Abstract

Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated Fatty Acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.

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