Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines

  • Eksp Onkol. 2004 Mar;26(1):20-3.
Radoslawa Nowak  1 ,  Maria Sawardo-Rochowska ,  Zygmunt Kazimierczuk ,  Roman J Nowak
Affiliations
  • 1. Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland. [email protected]
PMID: 15112575
Abstract

Aim: To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV Reverse Transcriptase on the growth of cultured normal and Cancer cells.

Methods: The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five Cancer cell lines have been tested. Cell viability was assessed by MTT assay.

Results: We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.

Conclusions: 2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other Telomerase inhibitors.

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