A fluorogenic and chromogenic probe that detects the esterase activity of trace copper(II)

  • Bioorg Med Chem Lett. 2005 Nov 1;15(21):4824-7. doi: 10.1016/j.bmcl.2005.07.042.
Radoslaw M Kierat  1 ,  Roland Krämer
Affiliations
  • 1. Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
PMID: 16140531 DOI: 10.1016/j.bmcl.2005.07.042
Abstract

Bis(picolinoyl)fluorescein, a new fluorogenic and chromogenic probe for monitoring the esterase activity of metal ions, has been synthesized. The probe is highly selective for Cu2+ and is applicable both to quantification of the free ion and detection of esterolytic activity of Cu bound to organic ligands or biomolecules, with a detection limit of 100 nM by fluorimetry.

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