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  3. Fluorescent Dye
  4. Bis(picolinoyl)fluorescein

Bis (picolinoyl) fluorescein is a Cu2+ esterase activity probe with both fluorogenic and chromogenic properties. Bis (picolinoyl) fluorescein enables quantitative analysis of free Cu2+, as well as detection of esterolytic activity of Cu bound to organic ligands or biomolecules.

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Bis(picolinoyl)fluorescein

Bis(picolinoyl)fluorescein Chemical Structure

CAS No. : 869343-71-5

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Bis(picolinoyl)fluorescein Related Antibodies

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Description

Bis (picolinoyl) fluorescein is a Cu2+ esterase activity probe with both fluorogenic and chromogenic properties. Bis (picolinoyl) fluorescein enables quantitative analysis of free Cu2+, as well as detection of esterolytic activity of Cu bound to organic ligands or biomolecules[1].

In Vitro

Bis(picolinoyl)fluorescein (Compound 1) (1-10 μM) acts as a highly selective chromogenic and fluorogenic probe for detecting the esterase activity of free and partially bound copper(II) ions in cell-free pH 7.0 buffer, with a fluorimetric detection limit of 0.1 μM copper(II)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bis(picolinoyl)fluorescein Related Antibodies
Molecular Weight

542.49

Formula

C32H18N2O7
CAS No.
SMILES

O=C(C1=CC=CC=N1)OC2=CC=C3C(OC4=C(C35OC(C6=CC=CC=C65)=O)C=CC(OC(C7=NC=CC=C7)=O)=C4)=C2
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Bis(picolinoyl)fluorescein Related Classifications

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bis(picolinoyl)fluorescein869343-71-5Fluorescent DyeDMF/pyridineCa2+esterase activityMg2+fluoresceinpicolinoyl chlorideNMRCu2+copper(II) ionsester hydrolysisInhibitorinhibitorinhibit

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