Chemo-enzymatic synthesis of allyl penta-N-acetyl-chitopentaose

  • Bioorg Med Chem Lett. 2006 Apr 1;16(7):2042-3. doi: 10.1016/j.bmcl.2005.12.055.
Gang-Liang Huang  1 ,  Da-Wei Zhang ,  Hong-Juan Zhao ,  Hou-Cheng Zhang ,  Peng-George Wang
Affiliations
  • 1. School of Life Science, Shandong University, Jinan City 250100, China. [email protected]
PMID: 16403625 DOI: 10.1016/j.bmcl.2005.12.055
Abstract

Cell density cultivation of recombinant Escherichia coli strains harboring the nodC gene (encoding chitooligosaccharide synthase) from Azorhizobium caulinodans has been previously described as a practical method for the preparation of gram-scale quantities of penta-N-acetyl-chitopentaose. We have now extended this method to the production of allylated derivative of penta-N-acetyl-chitopentaose by using allyl 2-acetamido-2-deoxy-beta-d-glucopyranoside (2) as the initial acceptor for the synthesis of target pentaoside in vivo.

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