FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

  • Growth Factors. 2007 Apr;25(2):71-6. doi: 10.1080/08977190701345200.
Jordi Farré  1 ,  Santiago Roura ,  Cristina Prat-Vidal ,  Carolina Soler-Botija ,  Anna Llach ,  Cristina E Molina ,  Leif Hove-Madsen ,  Jordi J Cairó ,  Francesc Gòdia ,  Ramon Bragós ,  Juan Cinca ,  Antoni Bayes-Genis
Affiliations
  • 1. Laboratory of Cell Physiology, Department of Cardiology, Institut Català de Ciencies Cardiovasculars, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Sant Antoni Ma Claret 167, 08025 Barcelona, Spain.
PMID: 17852409 DOI: 10.1080/08977190701345200
Abstract

Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.

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