Nonspecific effect of m7GMP on protein-RNA interactions

  • J Biol Chem. 1978 Oct 10;253(19):6630-2.
N Sonenberg ,  A J Shatkin
PMID: 211125
Abstract

Cap Analogs m7GMP and m7GDP inhibit binding of eukaryotic initiation factors to reovirus capped mRNA but also inhibit complex formation involving uncapped mRNA or 18 S rRNA. Furthermore, Escherichia coli DNA-dependent RNA polymerase binds 18 S rRNA and this interaction is also blocked by m7GMP. These results indicate that inhibition by Cap Analogs is not a stringent test for putative cap-specific binding between proteins and mRNA.

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