A new tool to assess ceramide bioactivity: 6-bromo-7-hydroxycoumarinyl-caged ceramide

  • Chem Commun (Camb). 2011 Aug 28;47(32):9236-8. doi: 10.1039/c1cc12345a.
Young Ah Kim  1 ,  Daniel M Carter Ramirez ,  Willard J Costain ,  Linda J Johnston ,  Robert Bittman
Affiliations
  • 1. Department of Chemistry and Biochemistry, Queens College of The City University of New York, Flushing, NY 11367, USA.
PMID: 21761060 DOI: 10.1039/c1cc12345a
Abstract

The bioactivity of natural, long-chain ceramides has until now been studied after its delivery to cells in organic solvent mixtures containing dodecane. We have synthesized ceramides conjugated to a (6-bromo-7-hydroxycoumarin-4-yl)methyl group. The photocaged ceramide is efficiently released with 350 nm light in aqueous solution at neutral pH, thus providing a promising new tool to study ceramide's properties.

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