Antimalarial β-carboline and indolactam alkaloids from Marinactinospora thermotolerans, a deep sea isolate

  • J Nat Prod. 2011 Oct 28;74(10):2122-7. doi: 10.1021/np200399t.
Hongbo Huang  1 ,  Yueliang Yao ,  Zhengxiang He ,  Tingting Yang ,  Junying Ma ,  Xinpeng Tian ,  Yayong Li ,  Caiguo Huang ,  Xiaoping Chen ,  Wenjun Li ,  Si Zhang ,  Changsheng Zhang ,  Jianhua Ju
Affiliations
  • 1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Rd., Guangzhou 510301, People's Republic of China.
PMID: 21977916 DOI: 10.1021/np200399t
Abstract

Four new β-carboline Alkaloids, designated marinacarbolines A-D (1-4), two new indolactam Alkaloids, 13-N-demethyl-methylpendolmycin (5) and methylpendolmycin-14-O-α-glucoside (6), and the three known compounds 1-acetyl-β-carboline (7), methylpendolmycin (8), and pendolmycin (9) were obtained from the fermentation broth of Marinactinospora thermotolerans SCSIO 00652, a new actinomycete belonging to the family Nocardiopsaceae. Their structures were elucidated by extensive MS and 1D and 2D NMR spectroscopic data analyses. The structure of compound 1 was further confirmed by single-crystal X-ray crystallography. The new compounds 1-6 were inactive against a panel of eight tumor cell lines (IC50>50 μM) but exhibited antiplasmodial activities against Plasmodium falciparum lines 3D7 and Dd2, with IC50 values ranging from 1.92 to 36.03 μM.