Thiobencarb herbicide reduces growth, photosynthetic activity, and amount of Rieske iron-sulfur protein in the diatom Thalassiosira pseudonana

  • J Biochem Mol Toxicol. 2013 Sep;27(9):437-44. doi: 10.1002/jbt.21505.
Yohei Shimasaki  1 ,  Michito Tsuyama ,  Rumana Tasmin ,  Xuchun Qiu ,  Motoyuki Shimizu ,  Yusuke Sato ,  Yasuhiro Yamasaki ,  Yoko Kato-Unoki ,  Atsushi Nukata ,  Takashi Nakashima ,  Hirofumi Ichinose ,  Hiroyuki Wariishi ,  Tsuneo Honjo ,  Yuji Oshima
Affiliations
  • 1. Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan. [email protected]
PMID: 23801610 DOI: 10.1002/jbt.21505
Abstract

We investigated the effects of the Herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and Photosystem II activity (Fv /Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6 f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb.

Keywords
Proteomics; Rieske Iron-Sulfur Protein; Thalassiosira pseudonana; Thiobencarb.
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