Thiobencarb
Based on 1 Customer Validation
Thiobencarb is an herbicide, can reduce growth, photosynthetic activity, and amount of rieske iron-sulfur protein in the diatom Thalassiosira pseudonana.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 28249-77-6
- Formula: C12H16ClNOS
- Molecular Weight:257.79
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Human Metabolite Library
HY-L123
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Pesticide Compound Library
HY-L183
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
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Environmental Pollutant Compound Library
HY-L247
Biological Activity
Thiobencarb (0.078-5 mg/L, 72 h) concentrations producing 10%, 20%, and 50% growth inhibition are calculated to be 0.55, 0.79, and 1.26 mg/L, respectively[1].
Thiobencarb (5, 2.5 mg/L, 6 h) is drastically decreased in the Fv/Fm ratio, after exposure of cells for up to 6 h under light and in darkness and three representative inhibitors of the photosynthetic electron transport chain[1].
Thiobencarb (5 mg/L, 24 h) increase expression levels of 13 proteins significantly and those of 11 proteins decreased significantly in Thalassiosira pseudonana. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 28249-77-6
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Appearance Liquid (Density: 1.145-1.180 g/cm3)
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Molecular Weight 257.79
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Formula C12H16ClNOS
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Color Colorless to light yellow
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SMILES
O=C(SCC1=CC=C(Cl)C=C1)N(CC)CC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (387.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (596 KB)
- English - EN (596 KB)
- Français - FR (596 KB)
- Deutsch - DE (596 KB)
- Norwegian - NO (596 KB)
- Español - ES (596 KB)
- Swedish - SV (596 KB)
- Italian - IT (596 KB)
- Portuguese - PT (596 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8791 mL | 19.3956 mL | 38.7913 mL | 96.9782 mL |
| 5 mM | 0.7758 mL | 3.8791 mL | 7.7583 mL | 19.3956 mL | |
| 10 mM | 0.3879 mL | 1.9396 mL | 3.8791 mL | 9.6978 mL | |
| 15 mM | 0.2586 mL | 1.2930 mL | 2.5861 mL | 6.4652 mL | |
| 20 mM | 0.1940 mL | 0.9698 mL | 1.9396 mL | 4.8489 mL | |
| 25 mM | 0.1552 mL | 0.7758 mL | 1.5517 mL | 3.8791 mL | |
| 30 mM | 0.1293 mL | 0.6465 mL | 1.2930 mL | 3.2326 mL | |
| 40 mM | 0.0970 mL | 0.4849 mL | 0.9698 mL | 2.4245 mL | |
| 50 mM | 0.0776 mL | 0.3879 mL | 0.7758 mL | 1.9396 mL | |
| 60 mM | 0.0647 mL | 0.3233 mL | 0.6465 mL | 1.6163 mL | |
| 80 mM | 0.0485 mL | 0.2424 mL | 0.4849 mL | 1.2122 mL | |
| 100 mM | 0.0388 mL | 0.1940 mL | 0.3879 mL | 0.9698 mL |
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