Direct light-up of cAMP derivatives in living cells by click reactions

  • Molecules. 2013 Oct 17;18(10):12909-15. doi: 10.3390/molecules181012909.
Kenichiro Ito  1 ,  Hongshan Liu ,  Makoto Komiyama ,  Tetsuya Hayashi ,  Yan Xu
Affiliations
  • 1. Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. [email protected].
PMID: 24141242 DOI: 10.3390/molecules181012909
Abstract

8-Azidoadenosine 3',5'-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

Products