Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol

  • FEBS Lett. 2015 May 8;589(11):1272-7. doi: 10.1016/j.febslet.2015.03.032.
Suguru Komenoi  1 ,  Fumika Takemura  1 ,  Hiromichi Sakai  1 ,  Fumio Sakane  2
Affiliations
  • 1. Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
  • 2. Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. Electronic address: [email protected].
PMID: 25862496 DOI: 10.1016/j.febslet.2015.03.032
Abstract

Diacylglycerol kinase (DGK) η plays important roles in various patho-physiological events such as oncogenesis. In this study, we performed an enzymological characterization of DGKη splice variant 1 (DGKη1). The Km value for diacylglycerol was 0.14 mol%. Intriguingly, the Km value of DGKη1 for diacylglycerol was at least 9-fold lower than those of Other DGK isozymes including DGKα, indicating that DGKη1 is a high affinity isozyme for diacylglycerol. Therefore, DGKη1 is a unique DGK isozyme, which may function at particular membrane sites where only low concentrations of diacylglycerol are supplied.

Keywords
ATP; Diacylglycerol; Diacylglycerol kinase; High affinity; Isozyme.
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