Cytotoxic Clerodane Diterpenoids from the Leaves of Casearia grewiifolia

  • J Nat Prod. 2015 Nov 25;78(11):2726-30. doi: 10.1021/acs.jnatprod.5b00677.
Ha T T Nguyen  1 ,  Ngan B Truong  1 ,  Huong T M Doan  1 ,  Marc Litaudon  2 ,  Pascal Retailleau  2 ,  Thao T Do  3 ,  Hung V Nguyen  1 ,  Minh V Chau  1 ,  Cuong V Pham  1
Affiliations
  • 1. Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
  • 2. Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR 2301, Université Paris-Sud , 91198, Gif-sur-Yvette, France.
  • 3. Institute of Biotechnology of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
PMID: 26516994 DOI: 10.1021/acs.jnatprod.5b00677
Abstract

Two new clerodane Diterpenoids (1 and 2) and the known compound caseanigrescen D (3) were isolated from the leaves of Casearia grewiifolia by bioassay-guided fractionation. Their structures were determined by analyses of MS and 2D NMR data. The absolute configurations of 1 and 3 were established by analysis of X-ray diffraction data. Compounds 1-3 were evaluated for their cytotoxicity against four Cancer cell lines, KB (mouth epidermal carcinoma cells), HepG-2 (human liver hepatocellular carcinoma cells), LU-1 (human lung adenocarcinoma cells), and MCF-7 (human breast Cancer cells). Caseagrewifolin B (2) had inhibitory activity against KB and HepG-2 cell lines with IC50 values of 6.2 to 7.0 μM, respectively. When tested against the normal cells (NIH/3T3), caseagrewifolin B (2) exhibited a significant selective inhibition against Cancer cells in comparison with the normal cells. Caseanigrescen D (3) was cytotoxic against four Cancer cell lines; however it had no selective inhibition compared with normal cells.