Induction of PINK1/Parkin-Mediated Mitophagy

  • Methods Mol Biol. 2018:1759:9-17. doi: 10.1007/7651_2017_7.
Shigeto Sato  1 ,  Norihiko Furuya  2
Affiliations
  • 1. Department of Neurology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan.
  • 2. Department of Neurology, Juntendo University School of Medicine, Hongo, Bunkyo, Tokyo, Japan. [email protected].
PMID: 28361482 DOI: 10.1007/7651_2017_7
Abstract

PINK1/Parkin Mitophagy is a key mechanism to contribute mitochondrial quality control, and the defects are thought to be a cause of those Parkinson's Disease onsets. Upon loss of mitochondrial membrane potential, PINK1 and Parkin are activated to promote the proteasomal degradation of mitochondrial outer membrane proteins and selective elimination of damaged mitochondria by Autophagy. In this chapter, we describe the methods for induction of PINK1/Parkin-mediated Mitophagy in tissue culture cell lines.

Keywords
Mitochondrial membrane potential; PINK1; Parkin.