Effect of Aging on the Absorption of Small Peptides in Spontaneously Hypertensive Rats

  • J Agric Food Chem. 2017 Jul 26;65(29):5935-5943. doi: 10.1021/acs.jafc.7b01727.
Vu Thi Hanh  1 ,  Weilin Shen  1 ,  Mitsuru Tanaka  1 ,  Aino Siltari  2 ,  Riita Korpela  2 ,  Toshiro Matsui  1
Affiliations
  • 1. Faculty of Agriculture, Graduate School of Kyushu University , Fukuoka 812-8581, Japan.
  • 2. Pharmacology, Faculty of Medicine, University of Helsinki , P.O. Box 63, 00014 University of Helsinki, Finland.
PMID: 28594555 DOI: 10.1021/acs.jafc.7b01727
Abstract

In the present study, we aimed to evaluate the effect of aging on the absorption of small peptides in spontaneously hypertensive rats (SHRs). Three kinds of dipeptides, glycyl-sarcosine (Gly-Sar), Trp-His, and captopril (a dipeptidomimetic drug), a Gly-Sar-Sar tripeptide, a Gly-Sar-Sar-Sar tetrapeptide, and a Gly-Sar-Sar-Sar-Sar pentapeptide were administered at doses of 10 mg/kg each to 8- and 40-week-old SHRs. The peptides were all detected in their intact forms in the blood. There was a significantly promoted absorption of di/tripeptides in aged SHRs compared with young SHRs. In contrast, the absorption of tetra/pentapeptides was not affected by aging. PepT1 expression in the mid-jejunum was significantly increased in 40-week-old SHRs compared with 8-week-old SHRs, whereas aging did not alter the expression of claudin-1, a tight junction related protein. Thus, the present results suggest that SHR aging may enhance the absorption of di/tripeptides through the enhanced PepT1 transport route, although oligopeptides may be absorbed in an age-independent manner.

Keywords
PepT1; absorption; aging; peptide; tight junction.
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