The expression of detoxification genes in two maize cultivars by interaction of isoxadifen-ethyl and nicosulfuron
- Plant Physiol Biochem. 2018 Aug:129:101-108. doi: 10.1016/j.plaphy.2018.05.025.
- 1. Henan Key Laboratory of Crop Pest Control, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
- 2. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
- 3. Henan Key Laboratory of Crop Pest Control, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. Electronic address: [email protected].
Herbicide safeners protect crop Plants from Herbicide phytotoxicity, but an understanding of their molecular mechanisms is still lacking. We investigated the effects of the safener isoxadifen-ethyl and/or nicosulfuron on the expression of 10 genes, 8 glutathione transferases (GSTs), 1 glutathione transporter and 1 multidrug resistance protein gene in two maize cultivars. Nicosulfuron and isoxadifen-ethyl induce different detoxification enzyme genes. The expression analyses of the 10 genes revealed that most were expressed much higher in 'Zhengdan958' than those in 'Zhenghuangnuo No.2', both in control and in isoxadifen-ethyl- and/or nicosulfuron-treated Plants. The expression levels of ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 in two maize cultivars were up-regulated by isoxadifen-ethyl only, or in combination with nicosulfuron, whereas nicosulfuron down-regulated the expression of eight genes. Thus, ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 could be considered safener-responsive and may be the core genes responsible for isoxadifen-ethyl increasing the tolerance of maize to nicosulfuron.