Simultaneous Determination of Ten Bioactive Components in Raw and Processed Radix Dipsaci by UPLC-Q-TOF-MS

  • J Chromatogr Sci. 2019 Feb 1;57(2):122-129. doi: 10.1093/chromsci/bmy093.
Yi Tao  1 ,  Surun Huang  2 ,  Weidong Li  2 ,  Baochang Cai  2
Affiliations
  • 1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
  • 2. Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.
PMID: 30247532 DOI: 10.1093/chromsci/bmy093
Abstract

Radix Dipsaci is employed for treating bone fractures in traditional Chinese medicine. In the current, a quantitative approach was established for determination of ten bioactive components (loganic acid, loganin, 3,5-diCQA, 4-CQA, 3,4-diCQA, sweroside, dipsacussaponin B, dipsacoside A, asperosaponin V and asperosaponin VI) in raw and rice wine-sautéed Radix Dipsaci by using UPLC-Q-TOF-MS based approach. The sample handling procedure was optimized. Chromatographic separation was operated on a Thermo Syncronis AQ-C18 UPLC column with mobile phase consisting of 0.01% aqueous formic acid and acetonitrile. The method was validated in terms of linearity, precision and recovery tests. All calibration curves displayed good linearity (R2 > 0.9991). The limit of detection and limit of quantification of these components were from 0.007 to 0.032 μg/mL and from 0.020 to 0.095 μg/mL, respectively. The average recoveries of these components were from 96.7 to 102.7% with relative standard deviation values from 0.13 to 3.81% for Radix Dipsaci. The developed approach can be employed for quality control of raw and wine-sautéed Radix Dipsaci.

Products