Antiproliferative and apoptotic activities of 8-prenylnaringenin against human colon cancer cells

  • Life Sci. 2019 Sep 1;232:116633. doi: 10.1016/j.lfs.2019.116633.
Sanaz Koosha  1 ,  Zahurin Mohamed  2 ,  Ajantha Sinniah  3 ,  Zaridatul Aini Ibrahim  4 ,  Atefehalsadat Seyedan  1 ,  Mohammed A Alshawsh  5
Affiliations
  • 1. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • 2. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: [email protected].
  • 3. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: [email protected].
  • 4. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: [email protected].
  • 5. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: [email protected].
PMID: 31278947 DOI: 10.1016/j.lfs.2019.116633
Abstract

Aims: The compound 8-prenylnaringenin (8-PN) is a prenylflavonoid that can be isolated from hops and beer and has anti-cancer properties against breast Cancer. The aim of this study is to investigate the anti-proliferative and apoptotic activities of 8-PN against human colon Cancer HCT-116 cells.

Main methods: Colon Cancer HCT-116 cells were treated with 8-PN and subjected to MTT and acridine orange/propidium iodide (AO/PI) staining to investigate the cytotoxicity of 8-PN. Arrest of the cells at different phases of cell cycle was monitored in the presence of 8-PN. Moreover, the apoptotic effects of 8-PN was assessed via annexin V and Caspase activity assays and compared to the untreated cells.

Key findings: The findings showed that 8-PN revealed strong inhibitory effect against HCT-116 cells with an IC50 value of 23.83 ± 2.9 μg/ml after 48 h. However, at similar concentrations and experimental time-points, the compound did not show cytotoxic effect to non-cancerous colon cells (CCD-41). Annexin-V assay indicates that 38.5% and 14.4% of HCT-116 cells had entered early and late stages of Apoptosis, respectively after exposure of the cells to 8-PN for 48 h. Caspase activity assay illustrates that Apoptosis is activated through both intrinsic and extrinsic pathways. Moreover, flow cytometry cell cycle results indicate that treatment with 8-PN significantly arrested the HCT-116 cells at G0/G1 phase.

Significance: These findings reveal that 8-PN has anti-proliferative activity against HCT-116 colon Cancer cells via induction of intrinsic and extrinsic pathway-mediated Apoptosis. Further investigations should be carried out to unravel the mechanistic pathways underlying these activities.

Keywords
8-Prenylnaringenin; Anti-proliferation; Apoptosis; Caspases; Cell cycle arrest; Colon cancer.
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