Indirect competitive enzyme-linked immunosorbent assay based on a broad-spectrum monoclonal antibody for tropane alkaloids detection in pig urine, pork and cereal flours

  • Food Chem. 2021 Feb 1;337:127617. doi: 10.1016/j.foodchem.2020.127617.
Zile Wang  1 ,  Pimiao Zheng  1 ,  Jianyi Wang  1 ,  Shuang He  1 ,  Zhenhui Ren  1 ,  Yanfang Zhang  1 ,  Jincheng Xiong  1 ,  Haiyang Jiang  2
Affiliations
  • 1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
  • 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China. Electronic address: [email protected].
PMID: 32799156 DOI: 10.1016/j.foodchem.2020.127617
Abstract

In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a broad-spectrum monoclonal antibody for Tropane Alkaloids (TAs) was established for the rapid screening of atropine, scopolamine, homatropine, apoatropine, anisodamine, anisodine and L-hyoscyamine residues in pig urine, pork and cereal flour samples through a simple sample preparation procedure. The half inhibitory concentrations of atropine, homatropine, L-hyoscyamine, apoatropine, scopolamine, anisodamine and anisodine were 0.05, 0.07, 0.14, 0.14, 0.24, 5.30 and 10.15 ng mL-1, respectivelyThe detection and quantitative limits of this method for TAs in samples were 0.18-73.18 and 0.44-74.77 μg kg-1. The spiked recoveries ranged from 69.88% to 147.93%, and the coefficient of variations were less than 14%. Good correlation (R2 = 0.9929) between the results of the ic-ELISA and the high performance liquid chromatography-tandem mass spectrometry support the reliability of the developed ic-ELISA method.

Keywords
Broad-spectrum monoclonal antibody; Cereal flours; Indirect competitive enzyme-linked immunosorbent assay; Pig urine; Pork; Tropane alkaloids.
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