Ganoderic acid B attenuates LPS-induced lung injury

  • Int Immunopharmacol. 2020 Nov:88:106990. doi: 10.1016/j.intimp.2020.106990.
Jiang Shi  1 ,  Huan Wang  2 ,  Jumin Liu  3 ,  Yang Zhang  4 ,  Junfang Luo  5 ,  Yan Li  6 ,  Chao Yang  7 ,  Junguang Jiang  8
Affiliations
  • 1. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 2. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 3. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 4. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 5. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 6. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
  • 7. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China.
  • 8. Department of Respiratory, the First Affiliated Hospital of Zhengzhou University, No.1 jianshe East Road, Zhengzhou, Henan 450052, China. Electronic address: [email protected].
PMID: 33182051 DOI: 10.1016/j.intimp.2020.106990
Abstract

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a serious respiratory disease, the mechanism is unclear. This paper revealed the mechanism of ganoderic acid B (BB) on lipopolysaccharide-induced pneumonia in mice. Pneumonia model was induced by LPS in mice and A549 cells. Lung dry/wet weight (W/D) and myeloperoxidase (MPO) activity in lung were examined. Lung histopathological changes was observed by HE staining. Superoxide dismutase (SOD), malondialdehyde (MDA) and proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in mice and A549 cells were detected. Rho/NF-κB pathway in mice and A549 cells were examined by Western Blot. BB significantly reduced W/D and MPO activity, restored lung histopathological changes. BB also increased SOD, decreased MDA, TNF-α, IL-1β and IL-6 in mice and A549 cells. In addition, BB inhibited Rho/NF-κB pathway in mice and A549 cells. BB has protective effect on LPS-induced pneumonia in mice, and its mechanism is related to the regulation of Rho/NF-κB signaling pathway.

Keywords
GanodericacidB; LPS; Lung injury; Rho/NF-κB.
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