Platelet-derived growth factor-AA promotes placental choriocarcinoma JAR cell proliferation via primary cilia
- Taiwan J Obstet Gynecol. 2022 Mar;61(2):299-305. doi: 10.1016/j.tjog.2022.02.018.
- 1. Department of Integrated Chinese and Western Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan; School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung, 840, Taiwan; Department of Chinese Medicine, E-Da Cancer Hospital, Kaohsiung, 824, Taiwan.
- 2. Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, 701, Taiwan.
- 3. Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 704, Taiwan.
- 4. Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 704, Taiwan. Electronic address: [email protected].
Objective: During early pregnancy, the proliferation placental cells is crucial for proper implantation and formation of maternal-fetal circulation. Platelet-derived growth factor-AA (PDGF-AA) has been detected in placenta during early pregnancy; however, the role of PDGF-AA in placental cell growth has not been studied extensively. Primary cilium, a centrosome-based cellular protrusion, is an signaling hub for regulating development and differentiation. Importantly, the receptor of PDGF-AA (Pdgfr-α) is detected in the primary cilium and primary cilia-mediated PDGF-AA signaling regulates development and differentiation. Here we would like to investigate whether PDGF-AA regulates placental cell growth and whether primary cilia play roles in this process.
Materials and methods: Human placental choriocarcinoma JAR cells were treated with PDGF-AA followed by examining cell growth. Primary cilia and subcellular localization of Pdgfr-α were observed by immunofluorescence staining. Manipulation of primary cilia was performed by treating cells with roscovitine or by transfecting cells with siRNA against IFT88.
Results: Here we showed that PDGF-AA induced JAR cell proliferation. In addition, JAR cells grew primary cilia where Pdgfr-α was detected. More importantly, pharmacological inhibition of primary cilia formation or depletion of cilia-related gene, IFT88, alleviated PDGF-AA induced JAR cell proliferation.
Conclusion: Thus, our study show that PDGF-AA facilitates human placental choriocarcinomaJARcell growth via primary cilia.