Notoginsenoside R1 induces oxidative stress and modulates LPS induced immune microenvironment of nasopharyngeal carcinoma

  • Int Immunopharmacol. 2022 Dec;113(Pt A):109323. doi: 10.1016/j.intimp.2022.109323.
Siwei Li  1 ,  Guoliang Pi  2 ,  Yong Zeng  3 ,  Changli Ruan  4 ,  Xiaosong He  5 ,  Xiaoxia Xiong  6 ,  Man Zhang  6 ,  Jiahua Zou  7 ,  Xinjun Liang  8
Affiliations
  • 1. Department of Oncology, Huanggang Central Hospital, 126 Qi'an Road, Huanggang City 438000, Hubei, China; Department of Radiation Oncology, the Affiliated Hospital of Guilin Medical University, Guilin 541004, Guangxi, China.
  • 2. Department of Radiation Oncology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430079, Hubei, China.
  • 3. Department of Oncology, Huanggang Central Hospital, 126 Qi'an Road, Huanggang City 438000, Hubei, China.
  • 4. Department of Radiotherapy, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
  • 5. Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Guilin Medical University, 15 Lequn Road, Guilin City, Guangxi Zhuang Autonomous Region, China.
  • 6. Department of Oncology, Huangzhou Hospital, Tongji Medical College of Huazhong University of Science and Technology, Huanggang City 438000, Hubei, China.
  • 7. Department of Oncology, Huanggang Central Hospital, 126 Qi'an Road, Huanggang City 438000, Hubei, China. Electronic address: [email protected].
  • 8. Department of Medical Oncology, Hubei Cancer Hospital, Tongji Medical College of Huazhong University of Science and Technology, 116 Zhuodaoquan South Road, Hongshan, Wuhan 430079, Hubei, China. Electronic address: [email protected].
PMID: 36279666 DOI: 10.1016/j.intimp.2022.109323
Abstract

Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor with high incidence. Notoginsenoside R1 (NGR1) is the main active compound of total Panax notoginseng saponin, and has multiple anti-tumor effects. This study aimed to investigate the effect and mechanism of NGR1 in NPC.

Materials: NPC cells were treated with different doses of NGR1. The NGR1 function in NPC was evaluated using Cell Counting Kit-8, Transwell, Western blot, flow cytometry, immunofluorescence assay, and quantitative Real-Time PCR. Meanwhile, the NGR1 mechanism in NPC was assessed by rescue experiments. Furthermore, the NGR1 function in vivo was determined by constructing an NPC xenotransplantation model, TUNEL, and immunohistochemistry assays.

Results: NGR1 repressed NPC cell growth and invasion but facilitated NPC cell Apoptosis and oxidative stress. Also, NGR1 alleviated inflammation in NPC cells. Mechanistically, NGR1 restrained NPC cell growth and induced oxidative stress in NPC cells, while these effects were abolished after lipopolysaccharide (an activator of the TRAF6/NF-κB pathway) treatment, implying that NGR1 reduced NPC cell growth and induced oxidative stress in NPC cells by the inactivation of TRAF6/NF-κB axis. Moreover, in vivo studies further proved the palliative effect of NGR1 on NPC.

Conclusion: NGR1 inhibited NPC cell growth and induced oxidative stress in NPC cells by inactivating TRAF6/NF-κB axis.

Keywords
Nasopharyngeal carcinoma; Notoginsenoside R1; Oxidative stress; TRAF6/NF-κB.
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