Starch from Pueraria lobata and the amylose fraction alleviates dextran sodium sulfate induced colitis in mice

  • Carbohydr Polym. 2023 Feb 15:302:120329. doi: 10.1016/j.carbpol.2022.120329.
Yifei Yang  1 ,  Mingxing Li  2 ,  Qingsong Liu  3 ,  Qianyun Zhao  1 ,  Jiuping Zeng  1 ,  Qin Wang  1 ,  Yueshui Zhao  2 ,  Fukuan Du  2 ,  Yu Chen  2 ,  Jing Shen  2 ,  Haoming Luo  1 ,  Shengpeng Wang  4 ,  Wanping Li  5 ,  Meijuan Chen  5 ,  Xiaobing Li  5 ,  Fang Wang  5 ,  Yuhong Sun  5 ,  Li Gu  5 ,  Zhangang Xiao  6 ,  Yu Du  7 ,  Xu Wu  8
Affiliations
  • 1. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China; Cell Therapy & Cell Drugs of Luzhou Key Laboratory, 646000 Luzhou, Sichuan, China.
  • 2. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China; Cell Therapy & Cell Drugs of Luzhou Key Laboratory, 646000 Luzhou, Sichuan, China; South Sichuan Institute of Translational Medicine, 646000 Luzhou, Sichuan, China.
  • 3. The First People's Hospital of Neijiang, 641000 Neijiang, Sichuan, China.
  • 4. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao.
  • 5. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China.
  • 6. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China; Department of Oncology, Affiliated Hospital of Southwest Medical University, 646000 Luzhou, Sichuan, China. Electronic address: [email protected].
  • 7. Medical Cosmetology Center, Affiliated Hospital of Traditional Chinese Medicine, Southwest Medical University, 646000 Luzhou, Sichuan, China. Electronic address: [email protected].
  • 8. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China; Cell Therapy & Cell Drugs of Luzhou Key Laboratory, 646000 Luzhou, Sichuan, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao. Electronic address: [email protected].
PMID: 36604040 DOI: 10.1016/j.carbpol.2022.120329
Abstract

Starch from Pueraria lobata (PLS) had polyhedral or spherical granules, displaying a bimodal size distribution within 0.6-30 μm. It showed a trimodal distribution of different molecular weight peaks, with amylose fraction of 18.2 %. PLS had a high crystallinity degree of 37.76 % and consisted of C-type starch, which gelatinized at 64.46-79.61 °C, with a high range of gelatinization (15.15 °C) and high enthalpy (13.98 J/g). A 21-day supplementation of PLS presented a regulative effect on gut microbiota in normal mice, and alleviated DSS-induced murine colitis through attenuating colonic inflammation, maintaining barrier function, preventing gut dysbiosis, increasing the short-chain fatty acids production and inhibiting NF-κB/IL-1β axis. The protective effect of PLS against colitis was in a gut microbiota-dependent manner. Notably, the amylose fraction was responsible for the prebiotic effect of PLS. The results would potentiate new application of PLS and the amylose fraction as functional prebiotics for prevention of colitis.

Keywords
Amylose; Colitis; Gut microbiota; Pueraria lobata; Short chain fatty acids; Starch.
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