Study on Calceolarioside A anti-Aβ25-35-induced Damage in SH-SY5Y Cells by Atomic Force Microscopy

  • Chem Biodivers. 2023 May 11;e202300430. doi: 10.1002/cbdv.202300430.
Yujuan Chen  1 ,  Kaige Qu  2 ,  Ying Wang  2 ,  Qinghan Zhang  2 ,  Ying Xie  2 ,  Zuobing Wang  2
Affiliations
  • 1. Changchun University of Science and Technology, School of life science, No.7089 Weixing Road, 130021, Changchun, CHINA.
  • 2. Changchun University of Science and Technology, International Research Centre for Nano Handling and Manufacturing of China, No.7089 Weixing Road, Changchun, CHINA.
PMID: 37166365 DOI: 10.1002/cbdv.202300430
Abstract

Calceolarioside A is a phenylethyl glycoside compound, originally isolated from the bark of Fraxinus mandshurica Rupr. In this work, the protective effect of Calceolarioside A on beta Amyloid protein induced toxicity in SH-SY5Y cells was studied. DPPH experiment, MTT assay, SEM, Atomic force microscopy and Colony formation were used to study the activity of Calceolarioside A. The results in this article show that the survival rate of the cells with Calceolarioside A (20 - 40 mg/mL) was significantly higher than that of the model cells without Calceolarioside A. Calceolarioside A could protect SH-SY5Y cells by improving some parameters in cells, such as the cell height, Young's modulus, adhesion and branch. In summary, Calceolarioside A can reduce Aβ25-35-induced damage in SH-SY5Y cells. It can be a potential medicine to treatment with AD.

Keywords
Antioxidants, Atomic Force Microscopy, Aβ25-35, Calceolarioside A, SH-SY5Y Cells.
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