Pestanoid A, a Rearranged Pimarane Diterpenoid Osteoclastogenesis Inhibitor from a Marine Mesophotic Zone Chalinidae Sponge-Associated Fungus, Pestalotiopsis sp. NBUF145

  • J Nat Prod. 2024 Jan 26;87(1):160-165. doi: 10.1021/acs.jnatprod.3c00892.
Tingting Wang  1 ,  Yunping Feng  1 ,  Jian Huang  2 ,  Sitong Wu  3 ,  Kun Hu  4 ,  Jiabin Wu  1 ,  C Benjamin Naman  5 ,  Hong Wang  3 ,  Wenhan Lin  2 ,  Shan He  1  2
Affiliations
  • 1. Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center, Ningbo University, Ningbo 315211, Zhejiang, China.
  • 2. Ningbo Institute of Marine Medicine, Peking University, Ningbo 315832, Zhejiang, China.
  • 3. School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
  • 4. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China.
  • 5. Department of Science and Conservation, San Diego Botanic Garden, Encinitas, California 92024, United States.
PMID: 38194474 DOI: 10.1021/acs.jnatprod.3c00892
Abstract

One novel rearranged pimarane diterpenoid, pestanoid A (1), and two reported molecules, nodulisporenones A (2) and B (3), were discovered from Pestalotiopsis sp. NBUF145 fungus associated with a 62 m deep mesophotic ("twilight") zone Chalinidae Sponge. The structures of 1-3 were identified by spectrometry, spectroscopy, quantum-chemical calculations, and X-ray crystallography. Compounds 1 and 2 inhibited bone marrow monocyte osteoclastogenesis in vitro with the IC50 values 4.2 ± 0.2 μM and 3.0 ± 0.4 μM, respectively, without observed cytotoxicity. Both 1 and 2 suppressed the receptor activator of NF-kB ligand-induced MAPK and NF-κB signaling by inhibiting the phosphorylation of ERK1/2-JNK1/2-p38 MAPKs and NF-κB nuclear translocation.

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