Antibacterial efficacy of Solanum muricatum aiton metabolites against methicillin-resistant staphylococcus aureus: Insights into bioactive compounds and molecular mechanisms

  • PLoS One. 2025 Dec 26;20(12):e0338733. doi: 10.1371/journal.pone.0338733.
Sarah Samir  1 ,  Fathy A Behery  2  3 ,  Mohamed A Zarka  4 ,  Ruqaiah I Bedaiwi  5 ,  Hesham A Abou-Zied  6 ,  Usama Ramadan Abdelmohsen  7  8 ,  Rehab Mahmoud Abd El-Baky  9  10 ,  Mohamed A Mawhoup  10 ,  Mai Mahrous  10 ,  Reem E S Abdelnaem  7 ,  Gerhard Bringmann  11 ,  Abeer H Elmaidomy  12
Affiliations
  • 1. Departement of Pharmacognosy, Faculty of Pharmacy, Misr University for Science and Technology (MUST), 6th October city, Giza, Egypt.
  • 2. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
  • 3. Department of Pharmacy, College of Pharmacy, Nursing and Medical Sciences, Riyadh Elm University, Riyadh, Saudi Arabia.
  • 4. Department of Pharmacognosy, College of Pharmacy, The Islamic University, Najaf, Iraq.
  • 5. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia.
  • 6. Department of Medicinal Chemistry, Faculty of Pharmacy, Deraya University, Minia, Egypt.
  • 7. Deraya Center for Scientific Research, Deraya University, Minia, Egypt.
  • 8. Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • 9. Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • 10. Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia, Egypt.
  • 11. Institute of Organic Chemistry, University of Würzburg, Würzburg, Germany.
  • 12. Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
PMID: 41452877 DOI: 10.1371/journal.pone.0338733
Abstract

The incidence of methicillin-resistant Staphylococcus aureus (MRSA) has been steadily increasing in Ethiopia over the past few decades. As a result, the need for new Antibiotic classes has become imperative to combat the growing threat of multidrug-resistant bacteria, including MRSA. Phytochemical investigation of the aerial parts extract of the edible plant Solanum muricatum Aiton (F. Solanaceae) afforded eight known metabolites: kaempferol 3-O-gentiobioside (1), kaempferol 3-O-sambubioside (2), quercetin 3-O-rhamnoside (3), procyanidin A2 (4), procyanidin A2 3-O-glucoside (5), (2S)-2-hydroxy-3-[(9Z,12Z)-1-oxo-9,12-octadecadien-1-yl]oxy]propyl-O-β-D-galactopyranoside (6), palmitic acid (7), and linoleic acid (8). The structures of the isolated compounds were assigned by 1D and 2D NMR. The crude extract exhibited moderate anti-Staphylococcus activity (MIC = 196.8 µg/mL), while compound 1 (kaempferol 3-O-gentiobioside) showed the strongest inhibitory effect (MIC = 8.3 µM), followed by compounds 2 and 3 (MIC = 10.2 and 11.2 µM, respectively). These compounds significantly reduced MRSA biofilm formation by up to 75.09% at sub-MIC concentrations (p < 0.05). Checkerboard assays revealed synergistic interactions among compounds 1, 2, and 3 and between these compounds and gentamicin (FICI < 0.5), suggesting enhanced therapeutic potential when combined. An integrated computational approach combining protein-protein interaction (PPI) network analysis, molecular docking, and molecular dynamics (MD) simulations was employed. The PPI network analysis, constructed using the STRING and STITCH databases, revealed critical MRSA-associated targets and their interactions with bioactive compounds from S. muricatum. Network hub analysis identified key immune-regulatory and Antibacterial resistance-related proteins, suggesting potential intervention points. Molecular docking results identified kaempferol 3-gentiobioside (compound 1) as the most potent inhibitor of APH(3')-IIIa, with strong binding energy and interactions with key catalytic residues. Further 150 ns MD simulations confirmed the stability of the compound 1-APH(3')-IIIa complex, as evidenced by minimal RMSD fluctuations, sustained hydrogen bonding, stable protein compactness (Rg), and favorable potential energy values.

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