Pyrithiamine hydrobromide
Based on 1 publication(s) in Google Scholar
Pyrithiamine (hydrobromide) is an inhibitor of thiamine kinase and thiamine pyrophosphate kinase. Pyrithiamine (hydrobromide) competitively inhibits thiamine transport and reduces intracellular levels of thiamine and its phosphates. Pyrithiamine (hydrobromide) is used to study thiamine deficiency with polyneuritic symptoms and Wernicke's encephalopathy.
For research use only. We do not sell to patients.
- Purity: 96.61%
- CAS No.: 534-64-5
- Formula: C14H20Br2N4O
- Molecular Weight:420.14
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pyrithiamine hydrobromide
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Biological Activity
Description
In Vitro
Pyrithiamine (hydrobromide) (6.23-300 μg; 10-60 min) potently inhibits TPP synthesis by partially purified rat liver thiaminokinase in vitro, with a pyrithiamine:thiamine ratio of 1:1 causing ~31-35% inhibition and higher ratios causing near-complete inhibition[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Pyrithiamine (hydrobromide) (623 μg; oral; daily; 4 days) alongside 100 μg thiamine reduces tissue TPP levels more rapidly in pigeons than withholding thiamine, particularly in brain tissue[1].
Pyrithiamine hydrobromide (0.1-0.5 mg/kg; i.p.; daily; up to 26 days) induces dose- and time-dependent reductions in brain Cho/NAA ratios in thiamine-deficient rats, with no significant effect on Cr/NAA ratios, and higher doses produce neurotoxic signs more rapidly[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (adult male, 200-300 g, thiamine deficiency induced via thiamine-deficient chow plus daily pyrithiamine hydrobromide injections)[2]
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Dosage:0.1 mg/kg; 0.5 mg/kg
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Administration:i.p.; daily; up to 26 days (0.1 mg/kg); up to 14 days (0.5 mg/kg)
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Result:Decreased Cho/NAA ratio by 65% by Day 26, with no significant change in Cr/NAA ratio, for 0.1 mg/kg dose.
Decreased Cho/NAA ratio by 60% by Day 14; reduced Cho/NAA from baseline 0.69 to 0.40 by Day 12, and to 0.29 by Day 14, with no significant change in Cr/NAA ratio, for 0.5 mg/kg dose.
Induced anorexia, loss of righting reflex, and seizures by Day 14 for 0.5 mg/kg dose.
Chemical Information
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CAS No. 534-64-5
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Appearance Solid
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Molecular Weight 420.14
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Formula C14H20Br2N4O
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Color White to light yellow
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SMILES
CC1=[N+](C=CC=C1CCO)CC2=C(N=C(N=C2)C)N.[Br-].Br
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Structure Classification
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Initial Source
Saccharomyces cerevisiae
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (238.02 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (288 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.3802 mL | 11.9008 mL | 23.8016 mL | 59.5040 mL |
| 5 mM | 0.4760 mL | 2.3802 mL | 4.7603 mL | 11.9008 mL | |
| 10 mM | 0.2380 mL | 1.1901 mL | 2.3802 mL | 5.9504 mL | |
| 15 mM | 0.1587 mL | 0.7934 mL | 1.5868 mL | 3.9669 mL | |
| 20 mM | 0.1190 mL | 0.5950 mL | 1.1901 mL | 2.9752 mL | |
| 25 mM | 0.0952 mL | 0.4760 mL | 0.9521 mL | 2.3802 mL | |
| 30 mM | 0.0793 mL | 0.3967 mL | 0.7934 mL | 1.9835 mL | |
| 40 mM | 0.0595 mL | 0.2975 mL | 0.5950 mL | 1.4876 mL | |
| 50 mM | 0.0476 mL | 0.2380 mL | 0.4760 mL | 1.1901 mL | |
| 60 mM | 0.0397 mL | 0.1983 mL | 0.3967 mL | 0.9917 mL | |
| 80 mM | 0.0298 mL | 0.1488 mL | 0.2975 mL | 0.7438 mL | |
| 100 mM | 0.0238 mL | 0.1190 mL | 0.2380 mL | 0.5950 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Pyrithiamine hydrobromide Related Classifications
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