ACSS2 Protein, Mouse (His)
Based on 1 Customer Validation
ACSS2 Protein, Mouse (His) is an enzyme that converts acetate to acetyl-CoA, which supplies acetyl-CoA for tumor cells by capturing acetate as a carbon source under stressed conditions.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
ACSS2 Protein, Mouse (His) is an enzyme that converts acetate to acetyl-CoA, which supplies acetyl-CoA for tumor cells by capturing acetate as a carbon source under stressed conditions.
Background
Acetyl-CoA synthetase 2 (ACSS2), an enzyme that converts acetate to acetyl-CoA, imparts a competitive growth advantage under conditions of metabolic stress by enhancing the ability of cancer cells to use acetate as an additional nutritional source when other carbon sources are scarce or cannot be used to sustain lipid biomass production. The activity of ACSS2 contributes to cancer cell growth under low-oxygen and lipid-depleted conditions. The downregulation of ACSS2 expression is a metabolic hallmark of tumor progression and aggressive behavior in colorectal carcinoma[1][2].
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-6*His
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Accession
Q9QXG4 (M1-Q701)
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Molecular Construction
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N-term
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6*His
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AceCS (M1-Q701)
Accession # Q9QXG4 -
C-term
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Protein Length
Full Length
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Synonyms
ACSS2; Acetyl-Coenzyme A Synthetase 2 (ADP Forming); Prev. ACAS2; Acetyl-CoA Synthetase; AceCS; Acetate--CoA Ligase; ACS; AceCS1; Acetyl-Coenzyme A Synthetase, Cytoplasmic; CDNA FLJ53719, Highly Similar To Acetyl-Coenzyme A Synthetase, Cytoplasmic (EC6.2.
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AA Sequence
MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ
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Predicted Molecular Mass
82.9 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6%
trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Zachary T Schug, et al. Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress. Cancer Cell. 2015 Jan 12;27(1):57-71. [Content Brief]
[2]. Jeong Mo Bae, et al. Downregulation of acetyl-CoA synthetase 2 is a metabolic hallmark of tumor progression and aggressiveness in colorectal carcinoma. Mod Pathol. 2017 Feb;30(2):267-277. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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