AGO1 Protein, Human (sf9, His)
Based on 1 Customer Validation
The AGO1 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs) and inhibiting the translation of complementary mRNAs.Despite lacking endonuclease activity, AGO1 does not cleave target mRNA.AGO1 Protein, Human (sf9, His) is the recombinant human-derived AGO1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The AGO1 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs) and inhibiting the translation of complementary mRNAs.Despite lacking endonuclease activity, AGO1 does not cleave target mRNA.AGO1 Protein, Human (sf9, His) is the recombinant human-derived AGO1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
AGO1, a pivotal component in RNA-mediated gene silencing (RNAi), functions by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), thereby orchestrating the repression of mRNA translation through complementary base pairing. Notably, AGO1 lacks endonuclease activity, diverging from its counterparts, and does not participate in the cleavage of target mRNAs. Beyond post-transcriptional gene silencing, AGO1 is instrumental in transcriptional gene silencing (TGS), acting on promoter regions that exhibit complementarity to the bound short antigene RNAs (agRNAs). Its extensive network of molecular interactions involves key players such as DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1, YBX1, LIMD1, WTIP, AJUBA, APOBEC3F, APOBEC3G, and APOBEC3H. Moreover, AGO1's association with polysomes and messenger ribonucleoproteins (mNRPs) emphasizes its integral role in the dynamic regulation of gene expression at various stages.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
Q9UL18 (M1-A857)
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Molecular Construction
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N-term
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His
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AGO1 (M1-A857)
Accession # Q9UL18 -
C-term
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Protein Length
Full Length
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Synonyms
AGO1; EIF2C 1; Prev. EIF2C1; Golgi Endoplasmic Reticulum Protein 95 KDa; Argonaute RISC Catalytic Component 1; Argonaute 1, RISC Catalytic Component; Protein Argonaute-1; Argonaute 1; HAGO1; NEDLBAS; Eukaryotic Translation Initiation Factor 2C, 1; GERP95;
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AA Sequence
MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA
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Predicted Molecular Mass
99.5 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Gly, 0.5 PMSF, 0.5 mM EDTA, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (241 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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