ALCAM/CD166 Protein, Mouse (HEK293, Fc)
Based on 1 Customer Validation
ALCAM/CD166 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD166 expressed by HEK293 cells with C-Fc tag. CD166, a member of the immunoglobulin superfamily, is widely expressed in various tissues, such as neurons, fibroblasts, endothelial cells, and keratinocytes.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
ALCAM/CD166 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD166 expressed by HEK293 cells with C-Fc tag. CD166, a member of the immunoglobulin superfamily, is widely expressed in various tissues, such as neurons, fibroblasts, endothelial cells, and keratinocytes[1].
Background
The CD166/activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and is widely expressed in various tissues, such as neurons, fibroblasts, endothelial cells, and keratinocytes. ALCAM is involved in neurogenesis, angiogenesis, hematopoiesis, leukocyte trafficking, and hematopoietic stem cell maintenance in bone marrow niches[1].
Verified Bioactivity
Measured by its ability to block adhesion of HuT78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 (HY-P72922). Recombinant Mouse ALCAM/CD166 at 10 µg/mL blocked 90.19 % of HUT78 cell adhesion to immobilized recombinant human CD6 (10 µg/mL, 100 µL/well).
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-Fc
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Accession
Q61490 (W28-K527)
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Molecular Construction
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N-term
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ALCAM (W28-K527)
Accession # Q61490 -
Fc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ALCAM; CD166 Antigen; Activated Leukocyte Cell Adhesion Molecule; CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule
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AA Sequence
WYTVNSAYGDTIVMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSIANAKISDEKRFVCMLVTEDNVFEAPTLVKVFKQPSKPEIVNKAPFLETDQLKKLGDCISRDSYPDGNITWYRNGKVLQPVEGEVAILFKKEIDPGTQLYTVTSSLEYKTTRSDIQMPFTCSVTYYGPSGQKTIYSEQEIFDIYYPTEQVTIQVLPPKNAIKEGDNITLQCLGNGNPPPEEFMFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKRNMAASTTITVHYLDLSLNPSGEVTKQIGDTLPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIHWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADDISDENREKVNDQAK
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Predicted Molecular Mass
84.2 kDa
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Molecular Weight
Approximately 100-120 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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