Alkaline Phosphatase/ALPG Protein, Human (HEK293, His)
Based on 1 Customer Validation
Alkaline Phosphatase/ALPG protein is an enzyme that hydrolyzes phosphomonoesters under alkaline conditions to release inorganic phosphate. Alkaline Phosphatase is involved in processes such as bone mineralization and intestinal phosphate absorption, and exhibits anti-inflammatory activity. Alkaline Phosphatase/ALPG protein, Human (HEK293, His), is a recombinant Alkaline Phosphatase/ALPG protein expressed in HEK293 cells and tagged with a C-terminal His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Alkaline Phosphatase/ALPG protein is an enzyme that hydrolyzes phosphomonoesters under alkaline conditions to release inorganic phosphate. Alkaline Phosphatase is involved in processes such as bone mineralization and intestinal phosphate absorption, and exhibits anti-inflammatory activity. Alkaline Phosphatase/ALPG protein, Human (HEK293, His), is a recombinant Alkaline Phosphatase/ALPG protein expressed in HEK293 cells and tagged with a C-terminal His tag[1].
Background
Alkaline Phosphatase/ALPG protein is an enzyme that possesses the capability to hydrolyze a range of phosphate compounds. It exhibits the ability to break down various phosphate molecules through its enzymatic activity. Alkaline Phosphatase acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in an alkaline environment. Its enzymatic activity allows it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This versatile enzyme plays a vital role in various physiological processes, including bone mineralization, cellular signaling, and metabolism[1].
In Vitro
Alkaline Phosphatase is constitutively expressed in epithelial cell lines such as human renal cells and Henle intestinal cells, but shows no basal expression in cells like HeLa Henle and HeLa NIH[2].
In Vivo
Alkaline Phosphatase (Human; 200 µg; subcutaneous injection; 2-4 times) significantly reduces synovial macrophage infiltration and exhibits systemic anti-inflammatory effects in a rat arthritis model, with synergistic effects when combined with Methotrexate (HY-14519)[3].
Verified Bioactivity
1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Human Alkaline Phosphatase (Germ type) , His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of ≤8 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
P10696 (I20-D503)
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Molecular Construction
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N-term
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ALPG (I20-D503)
Accession # P10696 -
8*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ALPG; Alkaline Phosphatase Nagao Isozyme; Prev. ALPPL2; ALP-1; GCAP; Testicular And Thymus Alkaline Phosphatase; Germ Cell Alkaline Phosphatase; Placental-Like Alkaline Phosphatase; Alkaline Phosphatase, Placental Like 2; Placental Alkaline Phosphatase-Li
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AA Sequence
IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD
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Predicted Molecular Mass
53.7 kDa
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Molecular Weight
Approximately 58-68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Sharma U, et al. Alkaline phosphatase: an overview. Indian J Clin Biochem. 2014 Jul;29(3):269-78. [Content Brief]
[2]. COX RP, et al. Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture. J Gen Physiol. 1962 Jan;45(3):439-85. [Content Brief]
[3]. Chandrupatla DMSH, et al. Prophylactic and therapeutic activity of alkaline phosphatase in arthritic rats: single-agent effects of alkaline phosphatase and synergistic effects in combination with methotrexate. Transl Res. 2018 Sep;199:24-38. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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