1. Recombinant Proteins
  2. CD Antigens Enzymes & Regulators
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Hydrolases (EC 3)
  4. Glutamyl Aminopeptidase/CD249
  5. Aminopeptidase A Protein, Mouse (sf9, His)

Aminopeptidase A Protein, Mouse (sf9, His)

Cat. No.: HY-P72825
COA Handling Instructions

The aminopeptidase A protein is a key player in the regulation of central hypertension, exerting its effects by selectively cleaving the N-terminal acidic residues of peptides such as angiotensin II. The enzyme's preference for specific substrates is regulated by calcium levels, underscoring its critical role in controlling blood pressure and maintaining cardiovascular health. Aminopeptidase A Protein, Mouse (sf9, His) is the recombinant mouse-derived Aminopeptidase A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aminopeptidase A Protein, Mouse (sf9, His) is 905 a.a., with molecular weight of ~113 kDa.

For research use only. We do not sell to patients.

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Description

The aminopeptidase A protein is a key player in the regulation of central hypertension, exerting its effects by selectively cleaving the N-terminal acidic residues of peptides such as angiotensin II. The enzyme's preference for specific substrates is regulated by calcium levels, underscoring its critical role in controlling blood pressure and maintaining cardiovascular health. Aminopeptidase A Protein, Mouse (sf9, His) is the recombinant mouse-derived Aminopeptidase A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aminopeptidase A Protein, Mouse (sf9, His) is 905 a.a., with molecular weight of ~113 kDa.

Background

Aminopeptidase A protein is a key player in the regulation of central hypertension, exerting its influence by selectively cleaving N-terminal acidic residues from peptides such as angiotensin II. The enzyme's preference for specific substrates is modulated by calcium levels, underscoring its crucial role in controlling blood pressure and maintaining cardiovascular health. By cleaving peptides, aminopeptidase A protein affects the balance of vasoactive substances and ultimately contributes to the regulation of vascular tone and blood pressure. Understanding the intricate mechanisms and functional implications of this protein can provide valuable insights into the development of targeted therapies for hypertension-related conditions.

Biological Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin and the specific activity is > 200 pmoles/min/μg.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His

Accession

P16406 (R41-P945)

Gene ID

13809  [NCBI]

Molecular Construction
N-term
His
ENPEP (R41-P945)
Accession # P16406
C-term
Synonyms
Glutamyl aminopeptidase; EAP; AP-A; CD249; ENPEP
AA Sequence

RSCEQDTTPAPSQPPPEASTALPPQDQNVCPDSEDESGEWKNFRLPDFINPVHYDLEVKALMEEDRYTGIVTISVNLSKPTRDLWLHIRETKITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAAEDLAATSGDSVYRLTMEFKGWLNGSLVGFYKTTYMEDGQIRSIAATDHEPTDARKSFPCFDEPNKKSTYSISIIHPKEYSALSNMPEEKSEMVDDNWKKTTFVKSVPMSTYLVCFAVHRFTAIERKSRSGKPLKVYVQPNQKETAEYAANITQAVFDYFEDYFAMEYALPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNTVTMDWWDDLWLNEGFASFFEFLGVNHAEKDWQMLSQVLLEDVFPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLKKFQFANAKTSDFWDSLQEASNLPVKEVMDTWTSQMGYPVVTVSGRQNITQKRFLLDSKADPSQPPSELGYTWNIPVRWADNDNSRITVYNRLDKGGITLNANLSGDAFLKINPDHIGFYRVNYEGGTWDWIAEALSSNHTRFSAADRSSFIDDAFALARAQLLNYKIALNLTMYLKSEEDFLPWERVISSVSYIISMFEDDRELYPMIETYFQGQVKPVADLLGWQDTGSHITKLLRASILGFACKMGDREALGNASQLFDSWLKGSASIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVKLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKTMAWNWIQLNWDYLVSRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLNVNRQSIREWFASLP

Molecular Weight

Approximately 113 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Aminopeptidase A Protein, Mouse (sf9, His)
Cat. No.:
HY-P72825
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